CSNK1G2 Antibody (Antigen Affinity Purified)

CSNK1G2 Antibody ( Antigen Affinity Purified ) Datasheet

CSNK1G2 Antibody Product Information

• Immunogen: Recombinant Human CSNK1G2 protein (Catalog#12373-H07B)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human CSNK1G2 (rh CSNK1G2; Catalog#12373-H07B; P78368-1; Met 18-Lys 415). CSNK1G2 specific IgG was purified by Human CSNK1G2 affinity chromatography.

CSNK1G2 Antibody Usage Guide

• Specificity: Human CSNK1G2
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CSNK1G2 in WB. Using a DAB detection system, the detection limit for Human CSNK1G2 is approximately 2 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CSNK1G2. The detection limit for Human CSNK1G2 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CSNK1G2 Antibody Background

Casein kinase I gamma 2 isoform (CSNK1G2), a member of the large casein kinase I (CKI) family, may affect the development of brain, and associate with vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins, and participates in Wnt signaling. The Casein kinase 1 family of protein kinases are serine/threonine-selective enzymes that function as regulators of signal transduction pathways in most eukaryotic cell types. The CKI family includes several other isoforms (alpha, beta, gamma, and delta). CKIgamma2 (CSNK1G2) hyperphosphorylates the serine-repeat motif of CERT, thereby inactivating CERT and down-regulating the synthesis of sphingomyelin. MTA1s interacts with CSNK1G2 both in vitro and in vivo and colocalizes in the cytoplasm. MTA1s as a target of CSNK1G2, and provided new evidence to suggest that CSNK1G2 phosphorylates and modulates the functions of MTA1s. In mammalian cells, CSNK1G2 selectively and constitutively binds Smad3 but not Smad1, -2 or -4. CSNK1G2 is the first kinase known to mark activated Smad3 for destruction. Because the overexpression in human cancers, CSNK1G2 may act as an oncoprotein during tumorigenesis.

References

1. Yinan M. et al., 2004, Neurosci Lett. 368 (1): 2-6.
2. Davidson G. et al., 2005, Nature. 438 (7069): 867-72.
3. McKay RM. et al., 2001, Dev Biol. 235 (2): 388-96.
4. Tomishige N. et al., 2009, Mol Biol Cell. 20 (1): 348-57.
5. Mishra SK. et al., 2004, Oncogene. 23 (25): 4422-9.
6. Guo X. et al., 2008, Oncogene. 27 (58): 7235-47.