Human CSL Gene cDNA Clone / ORF Clone

Human CSL cDNA Clone Datasheet

CSL cDNA Clone Product Information

• Gene Bank Ref.ID: NM_005349.2
• cDNA Size: 1503
• Gene Synonym: SUH; csl; CBF1; KBF2; RBP-J; RBPJK; IGKJRB; RBPSUH; IGKJRB1; MGC61669; RBPJ
• Source: Human

CSL cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens recombination signal binding protein for immunoglobulin kappa J region DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 234 T/C, not causing the amino acid variation.

CSL cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CSL cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CSL cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACCACA CGGAGGGCTC GCCCGCGGAG GAGCCGCCTG CGCATGCTCC ATCGCCTGGG
61       AAATTTGGTG AGCGGCCTCC ACCTAAACGA CTTACTAGGG AAGCTATGCG AAATTATTTA
121      AAAGAGCGAG GGGATCAAAC AGTACTTATT CTTCATGCAA AAGTTGCACA GAAGTCATAT
181      GGAAATGAAA AAAGGTTTTT TTGCCCACCT CCTTGTGTAT ATCTTATGGG CAGCGGATGG
241      AAGAAAAAAA AAGAACAAAT GGAACGCGAT GGTTGTTCTG AACAAGAGTC TCAACCGTGT
301      GCATTTATTG GGATAGGAAA TAGTGACCAA GAAATGCAGC AGCTAAACTT GGAAGGAAAG
361      AACTATTGCA CAGCCAAAAC ATTGTATATA TCTGACTCAG ACAAGCGAAA GCACTTCATG
421      TTGTCTGTAA AGATGTTCTA TGGCAACAGT GATGACATTG GTGTGTTCCT CAGCAAGCGG
481      ATAAAAGTCA TCTCCAAACC TTCCAAAAAG AAGCAGTCAT TGAAAAATGC TGACTTATGC
541      ATTGCCTCAG GAACAAAGGT GGCTCTGTTT AATCGACTAC GATCCCAGAC AGTTAGTACC
601      AGATACTTGC ATGTAGAAGG AGGTAATTTT CATGCCAGTT CACAGCAGTG GGGAGCCTTT
661      TTTATTCATC TCTTGGATGA TGATGAATCA GAAGGAGAAG AATTCACAGT CCGAGATGGC
721      TACATCCATT ATGGACAAAC AGTCAAACTT GTGTGCTCAG TTACTGGCAT GGCACTCCCA
781      AGATTGATAA TTAGGAAAGT TGATAAGCAG ACCGCATTAT TGGATGCAGA TGATCCTGTG
841      TCACAACTCC ATAAATGTGC ATTTTACCTT AAGGATACAG AAAGAATGTA TTTGTGCCTT
901      TCTCAAGAAA GAATAATTCA ATTTCAGGCC ACTCCATGTC CAAAAGAACC AAATAAAGAG
961      ATGATAAATG ATGGCGCTTC CTGGACAATC ATTAGCACAG ATAAGGCAGA GTATACATTT
1021     TATGAGGGAA TGGGCCCTGT CCTTGCCCCA GTCACTCCTG TGCCTGTGGT AGAGAGCCTT
1081     CAGTTGAATG GCGGTGGGGA CGTAGCAATG CTTGAACTTA CAGGACAGAA TTTCACTCCA
1141     AATTTACGAG TGTGGTTTGG GGATGTAGAA GCTGAAACTA TGTACAGGTG TGGAGAGAGT
1201     ATGCTCTGTG TCGTCCCAGA CATTTCTGCA TTCCGAGAAG GTTGGAGATG GGTCCGGCAA
1261     CCAGTCCAGG TTCCAGTAAC TTTGGTCCGA AATGATGGAA TCATTTATTC CACCAGCCTT
1321     ACCTTTACCT ACACACCAGA ACCAGGGCCG CGGCCACATT GCAGTGCAGC AGGAGCAATC
1381     CTTCGAGCCA ATTCAAGCCA GGTGCCCCCT AACGAATCAA ACACAAACAG CGAGGGAAGT
1441     TACACAAACG CCAGCACAAA TTCAACCAGT GTCACATCAT CTACAGCCAC AGTGGTATCC
1501     TAA

 

Quality Control:cDNA sequencing results is available upon request.