Mouse CSK / C-Src kinase Protein (GST Tag)

C-src tyrosine kinase Protein Datasheet

CSK / C-Src kinase Protein Product Information

• Synonym: AW212630
• Protein Construction: A DNA sequence encoding the mouse CSK (P41241) (Met 1-Leu 450) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

CSK / C-Src kinase Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE
• Bio-activity: The specific activity was determined using a Poly (Glu:Tyr, 4:1) synthetic peptide substrate.
  The specific activity is 74 nmol/min/mg.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The secreted recombinant mouse CSK/GST chimera consists of 687 amino acids and has a calculated molecular mass of 78.5 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris,500mM NaCl, pH 8.0, 10%glycerol.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CSK / C-Src kinase protein

CSK / C-Src kinase Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CSK / C-Src kinase Protein Description

Tyrosine-protein kinase CSK, also known as C-Src kinase, Protein-tyrosine kinase CYL and CSK, is a cytoplasm and cell membrane protein which belongs to theprotein kinase superfamily, Tyr protein kinase family and CSK subfamily. SRC/Proto-oncogene c-Src is a protein tyrosine kinase that phosphorylates Src family member C-terminal tails, resulting in downregulation of Src family members. It is composed of three principal domains: an SH3 (Src homology 3) domain, an SH2 (Src homology 2) domain, and a catalytic domain. It is expressed in lung and macrophages. SRC/Proto-oncogene c-Src and SRC are two protein tyrosine kinases with similar amino acid sequences but very different structures and functions. The molecular basis of the specificity of SRC/Proto-oncogene c-Src targeting the SRC tail appears to involve both local and long-range interactions and illustrates the complexity of selective targeting in post-translational modification. SRC/Proto-oncogene c-Src can be regulated through coupling of the SH2 and kinase domains and that CSK provides a novel built-in activation mechanism for cytoplasmic tyrosine kinases. SRC/Proto-oncogene c-Src is an indispensable negative regulator for the Src family tyrosine kinases (SFKs) that play pivotal roles in various cell signalings. SRC/Proto-oncogene c-Src catalyzes a tail phosphorylation reaction on SRC and thereby restrains Src's activity and oncogenic potential.

References

1. Brauninger A. et al.,1992, Gene. 110: 205-11.
2. Sondhi D. et al., 1999, Biochemistry. 38 (34): 11147-55.
3. Ogawa A. et al., 2002, J Biol Chem. 277 (17): 14351-4.
4. Cole PA. et al., 2003, Curr Opin Chem Biol. 7 (5): 580-5.
5. Baumeister U. et al., 2005,EMBO J. 24 (9): 1686-95.