Mouse CSF2 Gene cDNA Clone / ORF Clone

CSF2 cDNA Clone Datasheet

CSF2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009969.4
• cDNA Size: 426
• Gene Synonym: Csfgm; Gm-CSf; MGI-IGM; MGC151255; MGC151257; Csf2
• Source: Mouse

CSF2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus colony stimulating factor 2 (granulocyte-macrophage) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

CSF2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CSF2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CSF2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGGCTGC AGAATTTACT TTTCCTGGGC ATTGTGGTCT ACAGCCTCTC AGCACCCACC
61       CGCTCACCCA TCACTGTCAC CCGGCCTTGG AAGCATGTAG AGGCCATCAA AGAAGCCCTG
121      AACCTCCTGG ATGACATGCC TGTCACGTTG AATGAAGAGG TAGAAGTCGT CTCTAACGAG
181      TTCTCCTTCA AGAAGCTAAC ATGTGTGCAG ACCCGCCTGA AGATATTCGA GCAGGGTCTA
241      CGGGGCAATT TCACCAAACT CAAGGGCGCC TTGAACATGA CAGCCAGCTA CTACCAGACA
301      TACTGCCCCC CAACTCCGGA AACGGACTGT GAAACACAAG TTACCACCTA TGCGGATTTC
361      ATAGACAGCC TTAAAACCTT TCTGACTGAT ATCCCCTTTG AATGCAAAAA ACCAGGCCAA
421      AAATGA

 

Quality Control:cDNA sequencing results is available upon request.