Mouse MCSFR/CSF1R Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
MG50059-M	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CSF1R cDNA Clone Datasheet

CSF1R cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001037859.2
cDNA Size:	2934
Gene Synonym:	Fms; CD115; Csfmr; Fim-2; CSF-1R; M-CSFR; AI323359; Csf1r?
Source:	Mouse

CSF1R cDNA Clone Description

cDNA Description:	ORF Clone of Mus musculus colony stimulating factor 1 receptor DNA.
Vector:	Cloning Vector: pMD18-T Simple Vector. Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for four point mutations:1659A/C resulting in the amino acid 553Arg substitution by Ser, 1695 T/C ,1929 T/C and 2211G/A not causing the amino acid variation.

CSF1R cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CSF1R cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

CSF1R cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGTTGG GGCCTCCTCT GGTCCTGCTG CTGGCCACAG TTTGGCATGG TCAGGGGGCC
61       CCTGTCATCG AGCCTAGTGG CCCAGAACTG GTTGTAGAGC CGGGTGAAAC GGTGACCCTG
121      CGATGTGTGA GCAATGGCAG TGTGGAATGG GATGGCCCCA TCTCTCCCTA CTGGACCTTG
181      GACCCTGAAT CTCCCGGAAG CACCCTGACC ACAAGAAACG CGACCTTCAA AAACACTGGG
241      ACCTACCGTT GTACCGAGCT TGAAGACCCC ATGGCAGGCA GTACCACCAT CCACTTGTAT
301      GTCAAAGATC CGGCCCACTC TTGGAATTTG CTGGCACAGG AGGTGACAGT GGTTGAGGGC
361      CAGGAAGCTG TGCTGCCCTG TCTGATCACT GACCCTGCAC TGAAGGACAG TGTCTCACTG
421      ATGCGTGAGG GGGGCAGGCA GGTCTTACGC AAAACGGTCT ACTTCTTCTC GCCATGGCGA
481      GGGTTCATTA TCCGCAAGGC TAAAGTCCTT GACAGCAATA CCTACGTGTG CAAGACCATG
541      GTGAATGGTA GGGAATCCAC CTCCACTGGC ATCTGGCTTA AGGTGAATCG AGTCCACCCA
601      GAGCCCCCAC AGATAAAATT GGAGCCTAGC AAGCTGGTGC GGATTCGAGG GGAGGCTGCG
661      CAGATCGTGT GCTCGGCCAC TAACGCCGAA GTGGGATTCA ACGTTATCCT CAAACGTGGA
721      GACACCAAGC TGGAAATCCC CCTAAACAGT GACTTCCAAG ATAACTATTA TAAAAAAGTC
781      CGGGCTCTCA GTCTCAACGC TGTGGACTTC CAAGACGCTG GCATATATTC TTGTGTGGCC
841      AGCAATGATG TTGGCACACG CACGGCCACC ATGAACTTCC AGGTGGTGGA GAGTGCCTAC
901      TTAAACTTGA CCTCTGAGCA GAGCCTCTTG CAGGAGGTGT CTGTGGGTGA CAGCCTCATC
961      CTCACGGTCC ATGCAGATGC CTACCCTAGC ATACAGCATT ACAACTGGAC CTACCTAGGT
1021     CCATTCTTTG AAGACCAGCG CAAGCTTGAG TTTATCACCC AAAGGGCCAT ATACAGGTAC
1081     ACATTCAAGC TCTTTCTGAA CCGTGTAAAG GCCTCAGAGG CGGGCCAGTA CTTCTTAATG
1141     GCACAAAACA AGGCAGGCTG GAATAATCTG ACCTTTGAGC TCACCCTGCG ATATCCCCCA
1201     GAGGTCAGTG TTACATGGAT GCCTGTGAAT GGCTCTGATG TCCTGTTCTG TGACGTCTCT
1261     GGGTACCCTC AGCCCAGCGT GACATGGATG GAGTGCAGGG GCCACACCGA TAGGTGTGAT
1321     GAAGCCCAGG CTTTGCAGGT TTGGAATGAC ACCCACCCTG AAGTCCTGAG TCAGAAGCCC
1381     TTCGACAAAG TGATCATTCA GAGCCAGCTG CCCATTGGGA CCTTAAAACA CAACATGACT
1441     TATTTTTGCA AAACCCACAA CAGTGTGGGT AACAGCTCTC AGTACTTCAG GGCCGTCTCC
1501     CTAGGACAAA GCAAGCAGCT CCCCGATGAG TCCCTCTTCA CTCCGGTGGT GGTGGCCTGT
1561     ATGTCTGTCA TGTCTCTGCT GGTGCTACTG CTGTTGCTGC TCTTGTACAA GTACAAGCAG
1621     AAGCCGAAGT ACCAGGTGCG CTGGAAGATC ATCGAGAGAT ACGAAGGCAA TAGCTACACC
1681     TTCATTGACC CTACTCAGTT GCCCTACAAT GAGAAGTGGG AGTTCCCTCG GAACAACCTG
1741     CAGTTTGGTA AGACTCTAGG AGCCGGTGCC TTTGGGAAGG TGGTGGAGGC TACAGCCTTT
1801     GGTCTGGGCA AAGAAGATGC AGTGCTGAAG GTGGCTGTGA AGATGCTAAA GTCCACGGCT
1861     CATGCTGATG AGAAGGAGGC CCTGATGTCA GAGCTGAAGA TCATGAGTCA CCTGGGACAG
1921     CACGAGAATA TAGTCAACCT CTTGGGAGCC TGTACTCACG GAGGACCTGT CCTGGTCATC
1981     ACTGAATACT GCTGCTATGG AGACCTACTC AACTTTCTCC GAAGGAAGGC CGAGGCTATG
2041     CTAGGACCCA GCCTGAGTCC TGGTCAGGAC TCCGAGGGAG ACTCCAGCTA CAAGAACATC
2101     CACCTGGAGA AGAAATATGT GCGCAGGGAC AGTGGCTTCT CCAGTCAGGG TGTAGACACC
2161     TACGTGGAGA TGAGGCCTGT CTCGACTTCT TCAAGTGACT CCTTCTTTAA GCAAGATCTG
2221     GACAAAGAGG CCAGCCGGCC CCTGGAGCTC TGGGACCTGC TCCACTTCTC CAGCCAAGTG
2281     GCTCAGGGCA TGGCCTTCCT TGCTTCTAAA AACTGCATCC ACCGGGACGT AGCAGCTCGA
2341     AACGTGCTGT TGACCAGCGG ACATGTGGCC AAGATTGGGG ACTTTGGACT GGCTAGGGAC
2401     ATCATGAATG ACTCCAACTA TGTTGTCAAG GGCAATGCCC GCCTGCCTGT AAAGTGGATG
2461     GCCCCAGAGA GCATCTTTGA CTGCGTCTAC ACAGTTCAGA GTGATGTGTG GTCCTACGGC
2521     ATCCTCCTCT GGGAGATCTT CTCGCTTGGT CTGAACCCCT ACCCCGGCAT CCTAGTGAAC
2581     AACAAGTTCT ACAAACTGGT GAAGGATGGA TACCAAATGG CCCAGCCTGT ATTTGCACCG
2641     AAGAACATAT ACAGCATCAT GCAGTCCTGC TGGGACCTGG AGCCTACCAG AAGACCCACC
2701     TTCCAACAGA TCTGCTTCCT CCTCCAGGAG CAGGCCCGAC TGGAGAGGAG AGACCAGGAC
2761     TATGCTAACC TGCCAAGCAG CGGTGGCAGC AGCGGCAGTG ACAGTGGTGG TGGCAGCAGC
2821     GGTGGCAGCA GCAGTGAGCC AGAAGAGGAG AGCTCCAGTG AACACCTGGC CTGCTGTGAG
2881     CCAGGGGACA TCGCCCAGCC CCTGCTGCAG CCTAACAACT ACCAGTTCTG CTGA

Quality Control:cDNA sequencing results is available upon request.

CSF1R/MCSF Receptor/CD115 related areas, pathways, and other information

Cytokine