CSF1R / MCSF Receptor / CD115 Antibody (Cytokine)

CSF1R / MCSF Receptor / CD115 Antibody Datasheet

CSF1R / MCSF Receptor / CD115 Antibody Product Information

• Immunogen: Recombinant Mouse CSF1R / MCSF Receptor / CD115 protein (Catalog#50059-M08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse CSF1R / MCSF Receptor / CD115 (rM CSF1R / MCSF Receptor / CD115; Catalog#50059-M08H; NP_001032948.2; Met 1-Ser 511). Total IgG was purified by Protein A affinity chromatography.

CSF1R / MCSF Receptor / CD115 Antibody Usage Guide

• Specificity: Mouse CSF1R / MCSF Receptor / CD115
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse CSF1R in WB.
• Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Mouse CSF1R. The detection limit for Mouse CSF1R is approximately 0.0049 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CSF1R / MCSF Receptor / CD115 Antibody Background

M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1, a cytokine involved in the proliferation, differentiation, and activation of macrophages. As a member of the typeIII subfamily of receptor tyrosine kinases (RTKs), M-CSF receptor is expressed mainly on cells of monocyte/macrophage lineage, and consists of an extracellular ligand-binding domain and a cytoplasmic protein tyrosine kinase (PTK) domain joined through a single transmembrane helix. Ligand binding induces the M-CSF receptor homodimerization, and leads to transphosphorylation of specific tyrosine and the subsequent signal transduction. This activated receptor exerts actions on multiple intracellular effectors which determine the magnitude and specificity of the biological responses, and thus mediates most if not all of the biological effects of the CSF1 cytokine, including cell growth, survival, differentiation and chemotaxis. Mutations of this gene have been associated with a predisposition to myeloid malignancy.

References

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