MCSFR / CSF1R Antibody (Rabbit Monoclonal Antibody) (Cytokine)

Macrophage Colony-Stimulating Factor 1 Receptor Antibody

M-CSFR / CSF1R / CD115 Antibody Product Information

• Immunogen: Recombinant human M-CSFR protein ( Catalog #10161-H08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 108
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human M-CSFR extracellular domain ( rh M-CSFR; Catalog #10161-H08H; Met 1- Glu 512; NP_005202.2 ).

M-CSFR / CSF1R / CD115 Antibody Usage Guide

• Specificity: Human M-CSFR / CSF1R / CD115
• No cross-reactivity in ELISA with
  

  Human GCSFR / CSF3R / CD114

  Human GM-CSF / CSF2

  Human GMCSFR-alpha / CSF2RA / CD116

  Human PDGFR-alpha / CD140a

  Human PDGFR-beta / CD140b

• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human MCSFR in WB. Using a DAB detection system, the detection limit for human MCSFR is approximately 0.005 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.1- 0.2 μg/mL with the appropriate secondary reagents to detect human MCSFR. The detection limit for human MCSFR is 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

M-CSFR / CSF1R / CD115 Antibody Background

Macrophage colony-stimulating factor 1 receptor, also known as colony stimulating factor 1 receptor, M-CFSR, CSF1R, and CD115, is a cytokine involved in the proliferation, differentiation, and activation of macrophages. As a member of the type â…¢ subfamily of receptor tyrosine kinases (RTKs), M-CFSR is expressed mainly on cells of monocyte / macrophage lineage, and consists of an extracellular ligand-binding domain and a cytoplasmic protein tyrosine kinase (PTK) domain joined through a single transmembrane helix. Ligand binding induces the M-CFSR homodimerization, and leads to trans-phosphorylation of specific tyrosine and the subsequent signal transduction. This activated receptor exerts actions on multiple intracellular effectors which determine the magnitude and specificity of the biological responses, and mediates most if not all of the biological effects of the CSF1 cytokine, including cell growth, survival, differentiation and chemotaxis. Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia.

References

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5. Pixley, FJ. et al., 2004, Trends.Cell.Biol. 14: 628-38.
6. Rovida, E. et al., 2001, J. Immunol. 166:1583-9.