CSF1R / M-CSFR / CD115 (aa 543-972) Protein (GST Tag) (Cytokine)

Macrophage colony-stimulating factor 1 receptor Protein Datasheet

CSF1R / M-CSFR / CD115 Protein Product Information

• Synonym: M-CSF R, CSF1R, FMS, CSFR, FIM2, C-FMS, CD115
• Protein Construction: A DNA sequence encoding the human CSF1R (NP_005202.2) cytoplasmic domain (Lys 543-Cys 972) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

CSF1R / M-CSFR / CD115 Protein QC Testing

• Purity: >82 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CSF1R (aa 543-972) /GST chimera consists of 667 amino acids and has a calculated molecular mass of 76 KDa. It migrates as an approximately 75 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.4, 20%glycerol, 0.3mM DTT
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CSF1R / M-CSFR / CD115 protein

CSF1R / M-CSFR / CD115 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CSF1R / M-CSFR / CD115 Protein Description

Macrophage colony-stimulating factor 1 receptor, also known as M-CSFR, CSF-1-R, and CD115, is a single-pass type I  membrane protein. M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1, a cytokine involved in the proliferation, differentiation, and activation of macrophages. As a member of the type I ¢ subfamily of receptor tyrosine kinases (RTKs), M-CSF receptor / CD115 is expressed mainly on cells of monocyte/macrophage lineage, and consists of an extracellular ligand-binding domain and a cytoplasmic protein tyrosine kinase (PTK) domain joined through a single transmembrane helix. Ligand binding induces the M-CSF receptor homodimerization, and leads to transphosphorylation of specific tyrosine and the subsequent signal transduction. This activated receptor exerts actions on multiple intracellular effectors which determine the magnitude and specificity of the biological responses, and thus mediates most if not all of the biological effects of the CSF1 cytokine, including cell growth, survival, differentiation and chemotaxis. Defects in M-CSFR are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of M-CSFR are found in microglia in Alzheimer's disease and after brain injuries. The increased receptor expression causes microglia to become more active.

References

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7. Rovida E. et al., 2001, J Immunol. 166: 1583-9.