Human CSF1 transcript variant 4 Gene cDNA Clone / ORF Clone (Cytokine)

CSF1 cDNA Clone Datasheet

CSF1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_172212.2
• cDNA Size: 1665
• Gene Synonym: MCSF; CSF-1
• Source: Human

CSF1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens colony stimulating factor 1 (macrophage) transcript variant 4 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1223 T/C resulting in the amino acid leu substitution by pro and 1506 G/A, 1567 C/A not causing the amino acid variation.

CSF1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CSF1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CSF1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG
61       TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
121      CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
181      ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
241      TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
301      GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
361      AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
421      ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
481      GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
541      CAAGATGTGG TGACCAAGCC TGATTGCAAC TGCCTGTACC CCAAAGCCAT CCCTAGCAGT
601      GACCCGGCCT CTGTCTCCCC TCATCAGCCC CTCGCCCCCT CCATGGCCCC TGTGGCTGGC
661      TTGACCTGGG AGGACTCTGA GGGAACTGAG GGCAGCTCCC TCTTGCCTGG TGAGCAGCCC
721      CTGCACACAG TGGATCCAGG CAGTGCCAAG CAGCGGCCAC CCAGGAGCAC CTGCCAGAGC
781      TTTGAGCCGC CAGAGACCCC AGTTGTCAAG GACAGCACCA TCGGTGGCTC ACCACAGCCT
841      CGCCCCTCTG TCGGGGCCTT CAACCCCGGG ATGGAGGATA TTCTTGACTC TGCAATGGGC
901      ACTAATTGGG TCCCAGAAGA AGCCTCTGGA GAGGCCAGTG AGATTCCCGT ACCCCAAGGG
961      ACAGAGCTTT CCCCCTCCAG GCCAGGAGGG GGCAGCATGC AGACAGAGCC CGCCAGACCC
1021     AGCAACTTCC TCTCAGCATC TTCTCCACTC CCTGCATCAG CAAAGGGCCA ACAGCCGGCA
1081     GATGTAACTG GTACCGCCTT GCCCAGGGTG GGCCCCGTGA GGCCCACTGG CCAGGACTGG
1141     AATCACACCC CCCAGAAGAC AGACCATCCA TCTGCCCTGC TCAGAGACCC CCCGGAGCCA
1201     GGCTCTCCCA GGATCTCATC ACCGCGCCCC CAGGGCCTCA GCAACCCCTC CACCCTCTCT
1261     GCTCAGCCAC AGCTTTCCAG AAGCCACTCC TCGGGCAGCG TGCTGCCCCT TGGGGAGCTG
1321     GAGGGCAGGA GGAGCACCAG GGATCGGAGG AGCCCCGCAG AGCCAGAAGG AGGACCAGCA
1381     AGTGAAGGGG CAGCCAGGCC CCTGCCCCGT TTTAACTCCG TTCCTTTGAC TGACACAGGC
1441     CATGAGAGGC AGTCCGAGGG ATCCTCCAGC CCGCAGCTCC AGGAGTCTGT CTTCCACCTG
1501     CTGGTACCCA GTGTCATCCT GGTCTTGCTG GCCGTCGGAG GCCTCTTGTT CTACAGGTGG
1561     AGGCGGAGGA GCCATCAAGA GCCTCAGAGA GCGGATTCTC CCTTGGAGCA ACCAGAGGGC
1621     AGCCCCCTGA CTCAGGATGA CAGACAGGTG GAACTGCCAG TGTAG

 

Quality Control:cDNA sequencing results is available upon request.