CSAG1 / CSAGE Protein

Chondrosarcoma associated gene 1 Protein Datasheet

CSAG1 / CSAGE Protein Product Information

• Synonym: CSAGE, CT24.1
• Protein Construction: A DNA sequence encoding the human CSAG1 (AAH59947.1) (Asp20-Pro78) was fused with the Fc region of human IgG₁ at the N-terminus.
• Source: Human
• Expression Host: Human Cells

CSAG1 / CSAGE Protein QC Testing

• Purity: > 90% as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu
• Molecular Mass: The recombinant human CSAG1/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 320 amino acids and has a calculated molecular mass of 35.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCSAG1/Fc monomer is approximately 37 kDa.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CSAG1 / CSAGE protein

CSAG1 / CSAGE Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CSAG1 / CSAGE Protein Description

Putative chondrosarcoma-associated gene 1 protein (CSAG1 or CASGE) is a member of a family of tumor antigens. This protein is expressed in chondrosarcomes, melanoma, but may also be expressed in normal tissues such as testis and cartilage. In other nomal tissues, CSAG1 / CSAGE has not been detected. It has been reported that CSAGE / CSAG1 belongs to the growing list of cancer / testic antigens as well. In all of the CSAGE positive samples, the melanoma antigen gene family was also expressed and this is the first report on the expression of cancer / testis antigens in chondrosarcoma. There are two isoforms produced by alternative splicing. One is CSAG1a, whose encoding gene has been chosen as the 'canonical' sequence. All positional information in CSAG1 refers to it and the other isoform is CSAG1b.

References

1. Lin C. et al., 2002, Gene. 285 (1-2): 269-78.
2. Levin L. CSage: Use of a Configurable Semanticallly Attributed Graph Editor as Framework for Editing and Visualization.
3. Lee K. et al., 2010, Low-Complexity Leakage-Based Carrier Frequency Offset Estimation Techniques for OFDMA Uplink Systems.