Rat CRP / C-reactive Protein (His Tag)

Rat C-reactive / CRP Protein

Rat C-reactive / CRP Protein Product Information

• Synonym: Aa1249, Ab1-341, Ab2-196, Ac1-114, Ac1262, Ac2-069, Ba2-693
• Protein Construction: A DNA sequence encoding the rat CRP ( NP_058792.1 ) extracellular domain ( Met 1 - Ser 230 ) was fused with a polyhistidine tag at the C-terminus
• Source: Rat
• Expression Host: Human Cells

Rat C-reactive / CRP Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 21
• Molecular Mass: The recombinant rat CRP comprises 221 amino acids and predicts a molecular mass of 24.6 kDa. The apparent molecular mass of the rat CRP is approximately 30 kDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CRP protein

Rat C-reactive / CRP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Rat C-reactive / CRP Protein Description

C-reactive protein (CRP) is synthesized by the liver in response to factors released by fat cells. It is a member of the pentraxin family of proteins. The levels of CRP rise in response to inflammation. Human C-reactive protein (CRP) is the classical acute phase reactant, the circulating concentration of which rises rapidly and extensively in a cytokine-mediated response to tissue injury, infection and inflammation. Serum CRP values are routinely measured, empirically, to detect and monitor many human diseases. However, CRP is likely to have important host defence, scavenging and metabolic functions through its capacity for calcium-dependent binding to exogenous and autologous molecules containing phosphocholine (PC) and then activating the classical complement pathway. CRP may also have pathogenic effects and the recent discovery of a prognostic association between increased CRP production and coronary atherothrombotic events is of particular interest.

References

1. Thompson D, et al., 1999, Structure. 7 (2): 169-77. 
2. Pradhan AD, et al., 2001, JAMA. 286 (3): 327-334. 
3. Pepys MB, et al., 2003, J Clin Invest. 111 (12): 1805-12.
4. Lau DC, et al., 2005, Am J Physiol Heart Circ Physiol. 288 (5): H2031-41.
5. Dehghan A, et al., 2007, Diabetes. 56 (3): 872.