Human CRH Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13664-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CRH cDNA Clone Datasheet

CRH cDNA Clone Product Information

Gene Bank Ref.ID:	BC011031
cDNA Size:	591
Gene Synonym:	CRF;CRH
Source:	Human

CRH cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens corticotropin releasing hormone DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CRH cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CRH cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CRH cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGGCTGC CGCTGCTTGT GTCCGCGGGA GTCCTGCTGG TGGCTCTCCT GCCCTGCCCG
61       CCATGCAGGG CGCTCCTGAG CCGCGGGCCG GTCCCGGGAG CTCGGCAGGC GCCGCAGCAC
121      CCTCAGCCCT TGGATTTCTT CCAGCCGCCG CCGCAGTCCG AGCAGCCCCA GCAGCCGCAG
181      GCTCGGCCGG TCCTGCTCCG CATGGGAGAG GAGTACTTCC TCCGCCTGGG GAACCTCAAC
241      AAGAGCCCGG CCGCTCCCCT TTCGCCCGCC TCCTCGCTCC TCGCCGGCGG CAGCGGCAGC
301      CGCCCTTCGC CGGAACAGGC GACCGCCAAC TTTTTCCGCG TGTTGCTGCA GCAGCTGCTG
361      CTGCCTCGGC GCTCGCTCGA CAGCCCCGCG GCTCTCGCGG AGCGCGGCGC TAGGAATGCC
421      CTCGGCGGCC ACCAGGAGGC ACCGGAGAGA GAAAGGCGGT CCGAGGAGCC TCCCATCTCC
481      CTGGATCTCA CCTTCCACCT CCTCCGGGAA GTCTTGGAAA TGGCCAGGGC CGAGCAGTTA
541      GCACAGCAAG CTCACAGCAA CAGGAAACTC ATGGAGATTA TTGGGAAATA A

Quality Control:cDNA sequencing results is available upon request.