Mouse CREG Gene cDNA Clone / ORF Clone

CREG cDNA Clone Datasheet

CREG cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011804.2
• cDNA Size: 663
• Gene Synonym: Creg; AA755314; Creg1
• Source: Mouse

CREG cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus cellular repressor of E1A-stimulated genes 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

CREG cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CREG cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CREG cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGCCC GTGCTCCTGA GCTCGCGCGT TCCCTGCTTG CTGCCCTTCT GGCGCCCGCG
61       CTGGTGGCAC TACTGGTGTC GCCGGCCTCG GGTCGCGGAG GCCGGGACCA CGGGGACTGG
121      GACGTGGACA GGCGATTGCC TCCGCTGCCA CCCCGCGAAG ACGGGCCGCG CGTGGCCCGC
181      TTCGTGACTC ACGTCTCAGA CTGGGGCTCG CTGGCCACTA TCTCCACAAT AAAGGAGGTG
241      CGCGGCTGGC CCTTCGCGGA CATCATCTCA ATCAGTGACG GTCCTCCGGG AGAAGGCACG
301      GGCGAGCCCT ACATGTACCT GAGTCCACTG CAGCAAGCCG TGAGCGACCT GCAGGAAAAT
361      CCAGAGGCTA CGCTGACTAT GTCTTTAGCA CAGACTGTCT ACTGTAGGAA TCATGGATTT
421      GATCCCCAGA GTCCCCTGTG TGTTCATATA ATGATGTCGG GAACTGTGAC CAAGGTGAAC
481      AAGACAGAAG AGGACTATGC AAGGGATTCG CTGTTTGTTC GACACCCTGA GATGAAGCAC
541      TGGCCTTCCA GCCATAACTG GTTCTTTGCT AAATTAAAAA TAAGCCGTAT CTGGGTCTTG
601      GACTACTTTG GTGGACCTAA AGTAGTGACA CCTGAAGAAT ATTTTAACGT CACGCTGCAG
661      TGA

 

Quality Control:cDNA sequencing results is available upon request.