CREB1 Protein (MBP Tag)

CAMP responsive element binding protein 1 Protein Datasheet

CREB1 Protein Product Information

• Synonym: CREB, MGC9284
• Protein Construction: A DNA sequence encoding the human CREB1 isoform B (P16220-2) (Met 1 –Asp 327) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.
• Source: Human
• Expression Host: E. Coli

CREB1 Protein QC Testing

• Purity: > 80 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CREB1/MBP fusion protein consists of 724 amino acids and has a calculated molecular mass of 78.7 KDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CREB1 protein

CREB1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CREB1 Protein Description

CAMP responsive element binding protein 1, also known as CREB-1, plays multiple functions as a transcription factor in gene regulation.This protein is a CREB transcription factor that is a member of the leucine zipper family of DNA-binding proteins. CREB1 proteins are also known to be expressed in several spliced isoforms that act as transcriptional activators or repressors. The activator isoforms, possessing the functional domains for kinase induction and for interaction with other transcriptional regulators, act as transcriptional activators.The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB-1 has a complex influence on behavioural responses to drugs of abuse which varies depending on the brain region in which it is expressed. CREB-1 is important for serotonin (5-HT)-induced long-term facilitation (LTF) of the sensorimotor synapse in Aplysia.

References

1. Liu RY. et al., 2011, J Neurosci. 431 (18): 68711.
2. Sadamoto H. et al., 2011, PLoS One. 6 (6): 20285.
3. Hoeffler JP. et al., 1988, Science. 242 (4884): 1430-3.