+86-400-890-9989
Product Catalog
Research Topics
Human CRB1 Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| HG13707-G |
|
YES |  |
|
|
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
 |
|||
CRB1 cDNA Clone Datasheet
CRB1 cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_201253.2 |
|
cDNA Size: |
4219 |
| Gene Synonym: |
LCA8; RP12;CRB1 |
| Source: | Human |
CRB1 cDNA Clone Description
|
cDNA Description: |
ORF Clone of Homo sapiens crumbs homolog 1 DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Same as Gene Bank Ref. ID sequence with the nucleotide 1297 T, 3221 T deletion mutation and the point mutation 4208 A/C resulting in the amino acid Glu substitution by Ala. |
CRB1 cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
CRB1 cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
CRB1 cDNA Clone Sequence and Quality Control
Sequence:
1 ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 61 TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 121 AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 181 AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 241 GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 301 GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 361 CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 421 GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 481 GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 541 AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 601 TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC 661 TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG 721 GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA 781 CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA 841 GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC 901 TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT 961 GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC 1021 AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG 1081 AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT 1141 TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA 1201 TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT 1261 TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTATGG AGGAAGGGAC TGTTCTGATA 1321 TTCTCCTGGG CTGTACCCAT CAGCAATGTC TAAATAATGG AACATGCATC CCTCACTTCC 1381 AAGATGGCCA GCATGGATTC AGCTGCCTGT GTCCATCTGG CTACACCGGG TCCCTGTGTG 1441 AAATCGCAAC CACACTTTCA TTTGAGGGCG ATGGCTTCCT GTGGGTCAAA AGTGGCTCAG 1501 TGACAACCAA GGGCTCAGTT TGTAACATAG CCCTCAGGTT TCAGACTGTT CAGCCAATGG 1561 CTCTTCTACT TTTCCGAAGC AACAGGGATG TGTTTGTGAA GCTGGAGCTG CTAAGTGGCT 1621 ACATTCACTT ATCAATTCAG GTCAATAATC AGTCAAAGGT GCTTCTGTTC ATTTCCCACA 1681 ACACCAGCGA TGGAGAGTGG CATTTCGTGG AGGTAATATT TGCAGAGGCT GTGACCCTTA 1741 CCTTAATCGA CGACTCCTGT AAGGAGAAAT GCATCGCGAA AGCTCCTACT CCACTTGAAA 1801 GTGATCAATC AATATGTGCT TTTCAGAACT CCTTTTTGGG TGGTTTACCA GTGGGAATGA 1861 CCAGCAATGG TGTTGCTCTG CTTAACTTCT ATAATATGCC ATCCACACCT TCGTTTGTAG 1921 GCTGTCTCCA AGACATTAAA ATTGATTGGA ATCACATTAC CCTGGAGAAC ATCTCGTCTG 1981 GCTCATCATT AAATGTCAAG GCAGGCTGTG TGAGAAAGGA TTGGTGTGAA AGCCAACCTT 2041 GTCAAAGCAG AGGACGCTGC ATCAACTTGT GGCTGAGTTA CCAGTGTGAC TGCCACAGGC 2101 CCTATGAAGG CCCCAACTGT CTGAGAGAGT ATGTGGCAGG CAGATTTGGC CAGGATGACT 2161 CCACTGGTTA TGTCATCTTT ACTCTTGATG AGAGCTATGG AGACACCATC AGCCTCTCCA 2221 TGTTTGTCCG AACGCTTCAA CCATCAGGCT TACTTCTAGC TTTGGAAAAC AGCACTTATC 2281 AATATATCCG TGTCTGGCTA GAGCGCGGCA GACTAGCAAT GCTGACTCCA AACTCTCCCA 2341 AATTAGTAGT AAAATTTGTT CTTAATGATG GAAATGTCCA CTTGATATCT TTGAAAATCA 2401 AGCCATATAA AATTGAACTG TATCAGTCTT CACAAAACCT AGGATTTATT TCTGCTTCTA 2461 CGTGGAAAAT CGAAAAGGGA GATGTCATCT ACATTGGTGG CCTACCTGAC AAGCAAGAGA 2521 CTGAACTTAA TGGTGGATTC TTCAAAGGCT GTATCCAAGA TGTAAGACTA AACAACCAAA 2581 ATCTGGAATT CTTTCCAAAT CCAACAAACA ATGCATCTCT CAATCCAGTT CTTGTCAATG 2641 TAACCCAAGG CTGTGCTGGA GACAACAGCT GCAAGTCCAA CCCCTGTCAC AATGGAGGTG 2701 TTTGCCATTC CCGGTGGGAT GACTTCTCCT GTTCCTGTCC TGCCCTCACA AGTGGGAAAG 2761 CCTGTGAGGA GGTTCAGTGG TGTGGATTCA GCCCGTGTCC TCACGGAGCC CAGTGCCAGC 2821 CGGTGCTTCA AGGATTTGAA TGTATTGCAA ATGCTGTTTT TAATGGACAA AGCGGTCAAA 2881 TATTATTCAG AAGCAATGGG AATATTACCA GAGAACTCAC CAATATCACA TTTGGTTTCA 2941 GAACAAGGGA TGCAAATGTA ATAATATTGC ATGCAGAAAA AGAGCCTGAA TTTCTTAATA 3001 TTAGCATTCA AGATTCCAGA TTATTCTTTC AATTGCAAAG TGGCAACAGC TTTTATATGC 3061 TAAGTCTGAC AAGTTTGCAG TCAGTGAATG ATGGCACATG GCACGAAGTG ACCCTTTCCA 3121 TGACAGACCC ACTGTCCCAG ACCTCCAGGT GGCAAATGGA AGTGGACAAC GAAACACCTT 3181 TTGTGACCAG CACAATTGCT ACTGGAAGCC TCAACTTTTG AAGGATAATA CAGATATTTA 3241 TGTGGGAGAC AGAGCTATTG ACAATATAAA GGGCCTGCAA GGGTGTCTAA GTACAATAGA 3301 AATCGGAGGC ATTTATCTCT CTTACTTTGA AAATGTTCAT GGTTTCATTA ATAAACCTCA 3361 GGAAGAGCAA TTTCTCAAAA TCTCTACCAA TTCAGTGGTC ACTGGCTGTT TGCAGTTAAA 3421 TGTCTGCAAC TCCAACCCCT GTTTGCATGG AGGAAACTGT GAAGACATCT ATAGCTCTTA 3481 TCATTGCTCC TGTCCCTTGG GATGGTCAGG GAAACACTGT GAACTCAACA TCGATGAATG 3541 CTTTTCAAAC CCCTGTATCC ATGGCAACTG CTCTGACAGA GTTGCAGCCT ACCACTGCAC 3601 ATGTGAGCCT GGATACACTG GTGTGAACTG TGAAGTGGAT ATAGACAACT GCCAGAGTCA 3661 CCAGTGTGCA AATGGAGCCA CCTGCATTAG TCATACTAAT GGCTATTCTT GCCTCTGTTT 3721 TGGAAATTTT ACAGGAAAAT TTTGCAGACA GAGCAGATTA CCCTCAACAG TCTGTGGGAA 3781 TGAGAAGACA AATCTCACTT GCTACAATGG AGGCAACTGC ACAGAGTTCC AGACTGAATT 3841 AAAATGTATG TGCCGGCCAG GTTTTACTGG AGAATGGTGT GAAAAGGACA TTGATGAGTG 3901 TGCCTCTGAT CCGTGTGTCA ATGGAGGTCT GTGCCAGGAC TTACTCAACA AATTCCAGTG 3961 CCTCTGTGAT GTTGCCTTTG CTGGCGAGCG CTGCGAGGTG GACTTGGCAG ATGACTTGAT 4021 CTCCGACATT TTCACCACTA TTGGCTCAGT GACTGTCGCC TTGTTACTGA TCCTCTTGCT 4081 GGCCATTGTT GCTTCTGTTG TCACCTCCAA CAAAAGGGCA ACTCAGGGAA CCTACAGCCC 4141 CAGCCGTCAG GAGAAGGAGG GCTCCCGAGT GGAAATGTGG AACTTGATGC CACCCCCTGC 4201 AATGGCGAGA CTGATTTAG
Quality Control:cDNA sequencing results is available upon request.
