|IP||0.1-0.5 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-CRABP2 rabbit monoclonal antibody at 1:500 dilution
Lane A: 293T Whole Cell Lysate
Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:16 kDa
Observed band size:14 kDa
CRABP2 was immunoprecipitated using:
Lane A:0.5 mg 293T Whole Cell Lysate
Lane B:0.5 mg Raji Whole Cell Lysate0.5 µL anti-CRABP2 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.Primary antibody:
Anti-CRABP2 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 16 kDa
Observed band size: 16 kDa
Cellular retinoic acid-binding protein 2, also known as Cellular retinoic acid-binding protein II, CRABP-II and CRABP2, is a protein which belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The predicted amino acid sequences of human CRABP1 and CRABP2 demonstrated a 99.3% and 93.5% identity to mouse CRABP1 and CRABP2, respectively. CRABP2 forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Expression of CRABP2, but not CRABP1 mRNA, was markedly increased (greater than 15-fold) by retinoic acid treatment of fibroblasts cultured from human skin, whereas no significant induction of CRABP2 mRNA was observed in human lung fibroblasts. CRABP2 transports retinoic acid to the nucleus. It regulates the access of retinoic acid to the nuclear retinoic acid receptors. CRABP2 is necessary for elastin induction by All-trans retinoic acid (ATRA) in MRC-5 cells. It is expressed at low levels in emphysema fibroblasts. This alteration in the retinoic acid signalling pathway in lung fibroblasts may contribute to the defect of alveolar repair in human pulmonary emphysema.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-CRABP2 Antibody (PE)||Rabbit||Monoclonal||FCM||11203-R004-P|
|Anti-CRABP2 Antibody (FITC)||Rabbit||Monoclonal||FCM||11203-R004-F|
|Anti-CRABP2 Antibody (PE)||Mouse||Monoclonal||FCM||11203-MM05-P|
|Anti-CRABP2 Antibody (FITC)||Mouse||Monoclonal||FCM||11203-MM05-F|