Anti-CRABP2 Antibody

Cat: 11203-R004
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Anti-CRABP2 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-CRABP2 Antibody
Validated applications
WB,FCM,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human CRABP2
Immunogen
Recombinant Human CRABP2 protein (Catalog#11203-H07E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CRABP2 (rh CRABP2; Catalog#11203-H07E; NP_001869.1; Pro2-Glu138).
Source
Monoclonal Rabbit IgG Clone #004
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CRABP2 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ICC-IF 1:100-1:500  
FCM 1:100-1:500  
IP 0.1-0.5 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CRABP2 Antibody (Rabbit Monoclonal antibody) Images
Human CRABP2 Immunofluorescence(IF) 2954
Immunofluorescence staining of Human CRABP2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human CRABP2 monoclonal antibody (1:300) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Human CRABP2 Western blot (WB) 13362

Anti-CRABP2 rabbit monoclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lane B: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:16 kDa

Observed band size:14 kDa

Human CRABP2 Immunoprecipitation(IP) 14810

CRABP2 was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

Lane B:0.5 mg Raji Whole Cell Lysate

0.5 µL anti-CRABP2 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-CRABP2 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 16 kDa

Observed band size: 16 kDa

Human CRABP2 Flow Cytometry (FC) 18773
Flow cytometric analysis of Human CRABP2 expression on MCF-7 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CRABP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-CRABP2 Antibody Alternative Names
Anti-CRABP-II Antibody;Anti-RBP6 Antibody
CRABP2 Background Information

Cellular retinoic acid-binding protein 2, also known as Cellular retinoic acid-binding protein II, CRABP-II and CRABP2, is a protein which belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The predicted amino acid sequences of human CRABP1 and CRABP2 demonstrated a 99.3% and 93.5% identity to mouse CRABP1 and CRABP2, respectively. CRABP2 forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Expression of CRABP2, but not CRABP1 mRNA, was markedly increased (greater than 15-fold) by retinoic acid treatment of fibroblasts cultured from human skin, whereas no significant induction of CRABP2 mRNA was observed in human lung fibroblasts. CRABP2 transports retinoic acid to the nucleus. It regulates the access of retinoic acid to the nuclear retinoic acid receptors. CRABP2 is necessary for elastin induction by All-trans retinoic acid (ATRA) in MRC-5 cells. It is expressed at low levels in emphysema fibroblasts. This alteration in the retinoic acid signalling pathway in lung fibroblasts may contribute to the defect of alveolar repair in human pulmonary emphysema.

Full Name
cellular retinoic acid binding protein 2
References
  • Deak,KL.et al., 2005,Birth Defects Res A Clin Mol Teratol.73 (11): 868-75.
  • Plantier, L. et al., 2008, Thorax  63 (11): 1012-7.
  • Calmon, M.F. et al., 2009, Neoplasia  11 (12): 1329-39.
  • Welch, I.D. et al., 2009, Arthritis Res Ther  11 (1): R14.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CRABP2 Antibody Rabbit Polyclonal WB,ELISA,IHC-P 50535-RP02
    Anti-CRABP2 Antibody Rabbit Monoclonal ELISA 50535-R101
    Anti-CRABP2 Antibody Rabbit Polyclonal ELISA 50535-RP01
    Anti-CRABP2 Antibody Rabbit Polyclonal ELISA 11203-RP01
    Anti-CRABP2 Antibody Rabbit Polyclonal WB,ELISA,IP 11203-RP02
    Anti-CRABP2 Antibody Mouse Monoclonal FCM,ICC/IF,IF 11203-MM05
    Anti-CRABP2 Antibody (PE) Rabbit Monoclonal FCM 11203-R004-P
    Anti-CRABP2 Antibody (FITC) Rabbit Monoclonal FCM 11203-R004-F
    Anti-CRABP2 Antibody Mouse Monoclonal IHC-P 11203-MM01
    Anti-CRABP2 Antibody Mouse Monoclonal WB,ELISA 11203-MM04
    Anti-CRABP2 Antibody (PE) Mouse Monoclonal FCM 11203-MM05-P
    Anti-CRABP2 Antibody Rabbit Monoclonal WB 11203-R016
    Anti-CRABP2 Antibody (FITC) Mouse Monoclonal FCM 11203-MM05-F
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