Mouse CPA2 / Carboxypeptidase-A2 Protein (His Tag)

Carboxypeptidase A2, pancreatic Protein Datasheet

CPA2 / Carboxypeptidase-A2 Protein Product Information

• Synonym: CPA2
• Protein Construction: A DNA sequence encoding the mouse CPA2 (Q504N0) (Met 1-Tyr 417) was expressed, with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

CPA2 / Carboxypeptidase-A2 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 17
• Molecular Mass: The secreted recombinant mouse CPA2 (pro form) comprises 412 amino acids and has a calculated molecular mass of 46.6 kDa. It migrates as an approximately 47 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CPA2 / Carboxypeptidase-A2 protein

CPA2 / Carboxypeptidase-A2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CPA2 / Carboxypeptidase-A2 Protein Description

Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.

References

1. Catasus L. et al., 1995, J Biol Chem. 270: 6651-7.
2. Aloy P. et al., 1998, Biol Chem. 379: 149-55.
3. Laethem RM. et al., 1996, Arch Biochem Biophys. 332: 8-18.