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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human COMP / THBS5 cDNA ORF Clone, expression ready, FLAG-tagged||HG10173-M-F|
|Human COMP / THBS5 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG10173-M-H|
|Human COMP / THBS5 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG10173-M-M|
|Human COMP / THBS5 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG10173-M-N|
|Human COMP / THBS5 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG10173-M-Y|
Cartilage Oligomeric Matrix Protein (COMP), also referred to as Thrombospondin-5, is a non-collagenous extracellular matrix (ECM) protein and belongs to the subgroup B of the thrombospondin protein family. This protein is expressed primarily in cartilage, ligament, and tendon, and binds to other ECM proteins such as collagen I, II and IX with high affinities depending on the divalent cations Zn2+ or Ni2+. COMP is a secreted glycoprotein that is important for growth plate organization and function. It is suggested to play a role in cell growth and development, and recent studies have revealed the possible mechanism that it protects cells against death by elevating members of the IAP (inhibitor of apoptosis protein) family of survival proteins. Mutations in COMP cause two skeletal dysplasias, pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia (EDM1), and up-regulated expression of COMP are observed in rheumatoid arthritis and certain carcinomas.