Rat COLEC12 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80394-G	Cloning vector ($195) pCMV / hygro ($445) pCMV / hygro - HA ($445) pCMV / hygro - His ($445) pCMV / hygro - Myc ($445) pCMV / hygro - FLAG ($445)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

COLEC12 cDNA Clone Datasheet

COLEC12 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001025721.1
cDNA Size:	2229
Gene Synonym:	MGC114569; Colec12
Source:	Rat

COLEC12 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus collectin sub-family member 12 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

COLEC12 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

COLEC12 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

COLEC12 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAAGACG ACTTTGCAGA GGAAGAGGAG GTGCAGTCCT TCGGTTACAA GAGGTTTGGT
61       ATTCAGGAGG GGACACAATG TACCAAATGT AAAAATAACT GGGCACTGAA GTTTTCGATT
121      ATATTATTAT ATGTTTTGTG TGCCTTACTG ACCATCACAG TAGCCATTTT GGGATATAAA
181      GTTGTAGAGA AAATGGACAC TGTCACAGAT GGCATGGAGA CATCTCGCCA GACTTACGAC
241      AACAAGCTCA TTGCTGTGGA AAGTGATCTG AAGAAATTAG GGGACCAAAC TGGGAAGAAA
301      GCTCTAAGCA CCAACTCTGA GCTTTCTACC TTCAGATCAG ATATTCTGGA TCTCCGTCAG
361      CAACTTCAGG AGATCACAGA AAAAACCAGT AAGAACAAAG ATATGCTAGA GAAGTTGCAA
421      GCAAATGGGG ACTCATTGGT TGATAGACAA AGTCAACTGA AGGAAACTCT GCAGAATAAT
481      TCTTTCCTCA TCACCACAGT CAACAAAACT CTCCAGGCAT ATAATGGCTA TGTCACGAAT
541      CTGCAACAAG ATACTAGTGT GCTCCAGGGC AATCTGCAGA GCCAGATGTA TTCTCAGAAT
601      GTGGTTATCA TGAACCTCAA CAACCTGAAC CTGACCCAGG TTCAGCAGAG GAACCTTATC
661      ACAAATCTGC AGCGATCTGT GGATGACACG AGCCTGGCTA TCCAGCAAAT TAAGAATGAC
721      TTCCAAAATC TGCAACAGGT TTTCCTTCAA GCCAAGAAGG ACACCGATTG GCTAAAGGAG
781      AAAGTACAGA GTTTGCAGAC ATTGGCTGCC AACAACTCTG CCCTGGCCAA AGCCAATAAT
841      GACACCCTGG AGGATATGAA TAACCAGCTC AGCTCTTTCA CAGGTCAGAT GGACAACATT
901      ACCACTATCT CACAGGCTAA CGAGCAGAGC ATGAAAGACC TTCAGGACTT ACACAAGGAT
961      ACAGAAAATA GGACAGCTGT CAAGTTCAGC CAACTTGAGG AACGCTTCCA GGTCTTTGAG
1021     ACAGATATTG TGAACATCAT TAACAACATC AGCTACACAG CCCATCACCT GAGGACGCTG
1081     ACCAGCAATC TGAATGATGT TAGGACCACT TGCACAGACA CCTTGACCAG ACACACGGAT
1141     GACCTGACCT CCTTGAATAA CACACTAGTC AACATCCGCT TGGATTCTAT TTCTCTCAGG
1201     ATGCAGCAAG ACATGATGAG GTCAAGGTTA GACACTGAAG TAGCCAACTT ATCAGTGGTT
1261     ATGGAAGAGA TGAAGCTGGT TGACTCCAAG CATGGTCAGC TCATCAAGAA CTTTACCATT
1321     CTACAGGGTC CTCCTGGCCC CAGAGGTCCA AAAGGTGACA GAGGATCTCA GGGACCGCCT
1381     GGTCCAACTG GCAACAAGGG ACAGAAAGGA GAGAAGGGAG AGCCTGGTCC ACCTGGCCCT
1441     GCGGGTGAGC GAGGCACAAT TGGACCAGTA GGCCCTCCTG GAGAGCGTGG CAGCAAAGGA
1501     TCCAAAGGCT CACAGGGTCC CAAAGGATCA CGTGGGTCCC CAGGAAAGCC TGGCCCTCAA
1561     GGGCCTAGTG GAGACCCTGG ACCACCAGGT CCACCAGGCA AGGATGGACT CCCTGGCCCT
1621     CAGGGCCCTC CTGGCTTCCA GGGACTACAG GGCACTGTGG GTGAACCTGG AGTACCTGGA
1681     CCTCGGGGGC TACCAGGCTT ACCAGGGGTG CCAGGCATGC CTGGGCCCAA GGGACCACCT
1741     GGCCCCCCAG GTCCCTCAGG AGCAATGGAG CCGTTGGCTC TGCAGAATGA ACCAACCCCA
1801     GCATCAGAGG TCAACGGATG TCCGCCTCAC TGGAAGAACT TCACAGATAA ATGTTACTAT
1861     TTTTCGGTGG AGAAAGAAAT TTTTGAAGAT GCTAAGCTTT TCTGTGAAGA CAAATCTTCC
1921     CATCTCGTTT TCATAAACTC AAGAGAGGAA CAGCAATGGA TAAAAAAGCA GACCATGGGG
1981     AGAGAGAGCC ACTGGATCGG CCTCACAGAC TCAGAGCAAG AAAGCGAGTG GAAGTGGCTA
2041     GATGGGACAC CTGTTGATTA CAAAAACTGG AAAGCTGGAC AACCCGACAA CTGGGGCAGT
2101     GGCCATGGGC CTGGAGAAGA CTGTGCTGGA TTGATTTATG CCGGGCAGTG GAATGACTTC
2161     CAGTGTGATG AAATCAATAA CTTCATTTGC GAAAAAGAAA GGGAGGCAGT ACCATCATCC
2221     ATATTATAA

Quality Control:cDNA sequencing results is available upon request.