Cynomolgus monkey COLEC10 Gene cDNA Clone / ORF Clone

COLEC10 cDNA Clone Datasheet

COLEC10 cDNA Clone Product Information

• cDNA Size: 834
• Gene Synonym: COLEC10
• Source: Cynomolgus monkey

COLEC10 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) collectin sub-family member 10 (C-type lectin) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 332 A/T resulting in the amino acid Glu substitution by Thr.
  Please check the sequence information before order.

COLEC10 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

COLEC10 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

COLEC10 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAATGGCC TTGCATCCTT GCTTCGAAGA AACCAATTTA TCCTCCTGGT ACTATTTCTT
61       TTGCAAATTC AGAGTCTGGG TCTGGATATC GATAGCCGTC CTACCGCTGA AGTCTGTGCC
121      ACACACACAA TTTCACCAGG ACCCAAAGGA GATGATGGTG AAAAAGGAGA TCCAGGAGAA
181      GAGGGAAAGC ATGGCAAAGT GGGACGCATG GGGCCGAAAG GAATTAAAGG AGAACTGGGT
241      GATATAGGAG ATCAGGGCAA TATTGGCAAG ACTGGGCCCA TTGGCAAGAA GGGTGACAAA
301      GGGGAAAAAG GTTTGCTTGG AATACCTGGA GTAAAAGGCA AAGCAGGTAC CGTCTGTGAT
361      TGTGGAAGAT ACCGGAAATT TGTTGGACAA CTGGATATTA GTATTGCTCG GCTCAAGACA
421      TCTATGAAAT TTGTGAAGAA TGTGATAGCA GGGATTAGGG AAACTGAAGA GAAATTCTAC
481      TACATCGTGC AGGAAGAGAA GAACTACAGG GAATCCCTAA CCCACTGCAG GATTCGGGGT
541      GGAATGCTAG CCATGCCCAA GGATGAAGCT GCCAACACAC TCATAGCTGA CTATGTTGCC
601      AAGAGTGGCT TCTTTCGGGT GTTCATTGGC GTGAATGACC TTGAAAGGGA GGGACAGTAC
661      GTGTTCACAG ACAACACTCC ACTGCAGAAC TATAGCAACT GGAATGAGGG GGAACCCAGC
721      GACCCCTATG GTCATGAGGA CTGTGTGGAG ATGCTGAGCT CTGGCCGATG GAATGACACA
781      GAGTGCCATC TTACCATGTA CTTTGTCTGT GAGTTTATCA AGAAGAAAAA GTAA

 

Quality Control:cDNA sequencing results is available upon request.