Rat COLEC10 Gene cDNA Clone / ORF Clone

COLEC10 cDNA Clone Datasheet

COLEC10 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001130541.1
• cDNA Size: 834
• Gene Synonym: Colec10
• Source: Rat

COLEC10 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus collectin sub-family member 10 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 732 C/T not causing the amino acid variation.

COLEC10 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

COLEC10 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

COLEC10 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAATGGCT GTAGAGTCCT GCTTCGAAGA AACCAGTTCA TCCTGCTGCT GCTGTTTCTC
61       TTGCACTTTC AGAGTCTGGG TCTGGATGTT GATGGTCGCT CTGCTGCAGA AGTCTGTGTC
121      ACACATACCA TTTCACCAGG ACCTAAAGGA GATAATGGTG AAAGAGGTGA CACAGGAGAA
181      GAAGGCAAGG ATGGCAAAGT GGGACGCCAA GGACCAAAAG GAGTTAAAGG AGAGCTGGGT
241      GATATGGGAG CCCAGGGCGA TATTGGCAAG TCTGGTCCTA TTGGCAAGAA GGGTGACAAA
301      GGAGAGAAAG GTCTGCTTGG GGTACCTGGA GAAAAAGGCA AGGCAGGTAC CATCTGCGAC
361      TGTGGCAGAT ACCGGAAAGT GGTTGGACAA CTGGATATTA GTGTTGCTCG TCTTAAGACA
421      TCAATGAAAT TCATCAAGAA TGTCATAGCA GGGATCCGGG AAACTGAAGA GAAATTCTAC
481      TACATTGTGC AGGAGGAAAA GAACTACAGG GAATCTCTGA CCCACTGCAG GATCCGGGGA
541      GGGATGCTGG CCATGCCTAA GGATGAAGTT GTTAACACTC TTATTGCTGA CTATGTCGCC
601      AAGAGTGGCT TCTTCAGAGT GTTCATTGGC GTGAATGACC TTGAGAAGGA GGGGCAATAT
661      GTGTTCACAG ACAACACTCC ATTGCAGAAC TACAGCAACT GGAAGGAGGG GGAGCCTAGT
721      GACCCCTATG GTCATGAGGA CTGTGTGGAG ATGCTGAGCT CTGGCAGGTG GAACGACACA
781      GAGTGTCATC TTACCATGTA TTTTGTCTGT GAGTTTGTCA AGAAGAAAAA TTAA

 

Quality Control:cDNA sequencing results is available upon request.