Human COL9A2 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13680-G	Cloning vector ($195) pCMV / hygro ($445) pCMV / hygro - HA ($445) pCMV / hygro - His ($445) pCMV / hygro - Myc ($445) pCMV / hygro - FLAG ($445)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

COL9A2 cDNA Clone Datasheet

COL9A2 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001852.3
cDNA Size:	2070
Gene Synonym:	MED; EDM2; STL5; DJ39G22.4; COL9A2
Source:	Human

COL9A2 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens collagen, type IX, alpha 2 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 259 G/C, 1003 C/G resulting in the amino acid Gly substitution by Arg, Leu substitution by Val.

COL9A2 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

COL9A2 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

COL9A2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC
61       GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG
121      CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC
181      CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC
241      GGGATTGATG GTTTAACTCG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC
301      AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT
361      CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG
421      GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG
481      GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGT GTCCAACCAA CTGTCCACCC
541      GGAATGAAAG GTCCCCCAGG GCTGCAGGGA GTGAAGGGGC ATGCGGGCAA ACGCGGGATT
601      CTGGGTGATC CTGGCCACCA GGGGAAGCCG GGTCCCAAGG GAGATGTGGG TGCCTCTGGA
661      GAGCAAGGCA TCCCTGGACC ACCGGGTCCC CAGGGCATCA GGGGCTACCC AGGCATGGCA
721      GGGCCCAAGG GAGAGACGGG CCCTCATGGA TATAAAGGCA TGGTGGGCGC TATCGGTGCC
781      ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG GGACCGCCAG GCCGAGCTGG GGAGAAGGGT
841      GACGAGGGCA GCCCAGGTAT TCGTGGACCC CAGGGGATCA CAGGCCCGAA AGGAGCAACG
901      GGCCCCCCAG GCATCAACGG CAAGGATGGG ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT
961      GCAGGACAGG CGGGACAGCC CGGAAGTCCA GGCCACCAGG GCGTAGCGGG TGTGCCAGGC
1021     CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT
1081     GGATTCTCTG GTCCCCCTGG GAAAGAGGGA GAGCCAGGGC CTCGAGGAGA AATTGGTCCC
1141     CAGGGCATCA TGGGACAGAA GGGTGACCAA GGCGAGAGGG GTCCAGTGGG GCAACCAGGC
1201     CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG CAGGGCCCCC CCGGAATTCC AGGGCCCCAA
1261     GGCTTGCCAG GCGTCAAAGG AGACAAGGGC TCCCCAGGGA AGACCGGGCC CCGCGGCAAA
1321     GTGGGTGACC CAGGGGTGGC CGGCCTCCCC GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC
1381     GAGCCGGGGC CCAAGGGACA GCAAGGAGTA CGTGGAGAAC CCGGCTACCC TGGCCCCAGC
1441     GGGGATGCGG GCGCCCCAGG GGTTCAGGGC TACCCTGGTC CCCCCGGCCC TCGAGGACTG
1501     GCCGGGAACC GAGGCGTGCC AGGACAGCCC GGGAGACAGG GCGTGGAGGG CCGGGATGCC
1561     ACTGACCAGC ACATCGTGGA TGTGGCGCTG AAGATGCTGC AAGAGCAACT GGCAGAGGTC
1621     GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT GCGGTGGGCA TGATGGGTCC TCCAGGACCT
1681     CCTGGGCCCC CTGGGTACCC AGGCAAGCAG GGCCCCCATG GGCACCCTGG CCCTCGGGGC
1741     GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG ATCGGCAACA CGGGGCCCAA GGGAAAACGT
1801     GGAGAGAAGG GTGATCCAGG AGAAGTGGGA CGGGGGCACC CCGGGATGCC TGGGCCCCCA
1861     GGGATCCCAG GACTCCCTGG CCGGCCTGGC CAGGCAATCA ACGGCAAGGA TGGAGATCGA
1921     GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT CGACCTGGCC TGCCAGGCCC CGTGGGGCTG
1981     CCGGGCTTCT GTGAACCTGC CGCCTGCCTT GGAGCTTCGG CCTATGCCTC TGCCCGCCTT
2041     ACAGAGCCTG GATCCATCAA GGGGCCTTGA

Quality Control:cDNA sequencing results is available upon request.