Human COL9A1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12231-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

COL9A1 cDNA Clone Datasheet

COL9A1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC015409
cDNA Size:	987
Gene Synonym:	MED; EDM6; STL4; DJ149L1.1.2；COL9A1
Source:	Human

COL9A1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens collagen, type IX, alpha 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

COL9A1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

COL9A1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

COL9A1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGACCT GCTGGAAAAT TCCAGTTTTC TTCTTTGTGT GCAGTTTCCT GGAACCCTGG
61       GCATCTGCAG CTGTCAAGCG TCGCCCCAGA TTCCCTGTCA ATTCCAATTC TAATGGTGGA
121      AATGAACTCT GTCCAAAGAT CAGGATTGGC CAAGATGACT TACCAGGGTT TGATCTGATC
181      TCTCAGTTCC AGGTAGATAA AGCAGCATCT AGAAGAGCTA TCCAGAGAGT AGTGGGATCA
241      GCTACATTGC AGGTGGCTTA CAAGTTGGGA AATAATGTAG ACTTCAGGAT TCCAACTAGG
301      AATTTATATC CCAGTGGACT GCCTGAAGAA TACTCCTTCT TGACGACGTT TCGAATGACT
361      GGAAGCACTC TCAAAAAGAA CTGGAACATT TGGCAGATTC AGGATTCCTC TGGGAAGGAG
421      CAAGTTGGCA TAAAGATTAA TGGCCAAACA CAATCTGTTG TATTTTCATA CAAGGGACTG
481      GATGGAAGTC TCCAAACAGC AGCCTTTTCG AATTTGTCCT CCTTGTTTGA TTCCCAGTGG
541      CATAAGATCA TGATTGGCGT GGAGAGGAGT AGTGCTACTC TTTTTGTTGA CTGCAACAGG
601      ATTGAATCTT TACCTATAAA GCCAAGAGGC CCAATTGACA TTGATGGCTT TGCTGTGCTG
661      GGAAAACTTG CAGATAATCC TCAAGTTTCT GTTCCATTTG AACTTCAATG GATGCTGATC
721      CATTGTGACC CCCTGCGGCC CAGGAGAGAA ACTTGCCATG AGCTGCCAGC CAGAATAACG
781      CCCAGCCAGA CCACCGACGA GAGAGGTCCC CCGGGTGAGC AGGGTCCTCC CGGGCCTCCG
841      GGCCCCCCTG GAGTTCCAGG CATCGATGGC ATCGACGGTG ACCGAGGTCC TAAGGGCCCC
901      CCGGGCCCCC CGGGTCCTGC AGGTGAACCG GGAAAGCCAG GAGCTCCAGG CAAGCCTGGC
961      ACACCTGGCG CTGATACCAG TCCTTGA

Quality Control:cDNA sequencing results is available upon request.