Human COL5A2 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13685-G	Cloning vector ($495) pCMV / hygro ($745) pCMV / hygro - HA ($745) pCMV / hygro - His ($745) pCMV / hygro - Myc ($745) pCMV / hygro - FLAG ($745)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

COL5A2 cDNA Clone Datasheet

COL5A2 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_000393.3
cDNA Size:	4500
Gene Synonym:	COL5A2
Source:	Human

COL5A2 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens collagen, type V, alpha 2 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

COL5A2 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

COL5A2 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

COL5A2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATGGCAA ACTGGGCGGA AGCAAGACCT CTCCTCATTC TTATTGTTTT ATTAGGGCAA
61       TTTGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC
121      TGCACTCAGA ATGGCCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGTCAG
181      ATCTGTGTCT GTGACAATGG AGCCATTCTC TGTGACAAGA TAGAATGCCA GGATGTGCTG
241      GACTGTGCCG ACCCTGTAAC GCCCCCTGGG GAATGCTGTC CTGTCTGTTC ACAAACACCT
301      GGAGGTGGCA ATACAAATTT TGGTAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA
361      GTGCCTGTTG TAACAGGCAT ACGTGGTCGT CCAGGACCGG CAGGACCTCC AGGATCACAG
421      GGACCAAGAG GAGAGCGAGG GCCAAAAGGA AGACCTGGCC CTCGTGGACC TCAGGGAATT
481      GATGGAGAAC CAGGTGTTCC TGGTCAACCT GGTGCTCCAG GACCTCCTGG ACATCCGTCC
541      CACCCAGGAC CCGATGGCTT GAGCAGGCCG TTTTCAGCTC AAATGGCTGG GTTGGATGAA
601      AAATCTGGAC TTGGGAGTCA AGTAGGACTA ATGCCTGGCT CTGTGGGTCC TGTTGGCCCA
661      AGGGGACCAC AGGGTTTACA AGGACAGCAA GGTGGTGCAG GACCTACAGG ACCTCCTGGT
721      GAACCTGGTG ATCCTGGACC AATGGGTCCG ATTGGTTCAC GTGGACCAGA GGGCCCTCCT
781      GGTAAACCTG GGGAAGATGG TGAACCTGGC AGAAATGGAA ATCCTGGTGA AGTGGGATTT
841      GCAGGATCTC CGGGAGCTCG TGGATTTCCT GGGGCTCCTG GTCTTCCAGG TCTGAAGGGT
901      CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCACC TGGTTCCAAG
961      GGTGAAGCTG GCCCCACTGG TCCAATGGGT GCCATGGGTC CTCTGGGTCC GAGGGGAATG
1021     CCAGGAGAGA GAGGGAGACT TGGGCCACAG GGTGCTCCTG GACAACGAGG TGCACATGGT
1081     ATGCCTGGAA AACCTGGACC AATGGGTCCT CTTGGGATAC CAGGCTCTTC TGGTTTTCCA
1141     GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCTACAGGGG CGCGAGGCCC TGAAGGTCCT
1201     CAGGGGCAGA GAGGTGAAAC TGGGCCCCCA GGTCCAGTTG GCTCTCCAGG TCTTCCTGGT
1261     GCAATAGGAA CTGATGGTAC TCCTGGTGCC AAAGGCCCAA CGGGCTCTCC GGGTACCTCT
1321     GGTCCTCCTG GCTCAGCAGG GCCTCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGTCCT
1381     CAGGGAATTC GAGGCCAACC GGGTGATCCA GGAGTTCCAG GTTTCAAAGG AGAAGCTGGC
1441     CCAAAAGGGG AACCAGGGCC ACATGGTATT CAGGGTCCGA TAGGCCCACC CGGTGAAGAA
1501     GGCAAAAGAG GTCCCAGAGG TGACCCAGGA ACAGTTGGTC CTCCAGGGCC AGTGGGAGAA
1561     AGGGGTGCTC CTGGCAATCG TGGTTTTCCA GGCTCTGATG GTTTACCTGG GCCAAAGGGT
1621     GCTCAAGGAG AACGGGGTCC TGTAGGTTCT TCAGGACCCA AAGGAAGCCA GGGGGATCCA
1681     GGACGTCCAG GGGAACCTGG GCTTCCAGGT GCTCGGGGTT TGACAGGAAA TCCTGGTGTT
1741     CAAGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCGCCAG GGGAAGATGG CCGTCCAGGT
1801     CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCCGGGAGCA TGGGCCTTCC AGGCCCCAAA
1861     GGTAGCAGTG GTGACCCTGG GAAACCTGGA GAAGCAGGAA ATGCTGGAGT TCCTGGGCAG
1921     AGGGGAGCTC CTGGAAAAGA TGGTGAAGTT GGTCCTTCTG GTCCTGTGGG CCCGCCGGGT
1981     CTAGCTGGTG AAAGAGGAGA ACAAGGACCT CCAGGCCCCA CAGGTTTTCA GGGGCTTCCT
2041     GGTCCTCCAG GGCCTCCTGG AGAAGGTGGA AAACCAGGTG ATCAAGGTGT TCCTGGAGAT
2101     CCCGGAGCAG TTGGCCCGTT AGGACCTAGA GGAGAACGAG GAAATCCTGG GGAAAGAGGA
2161     GAACCTGGGA TAACTGGACT CCCTGGTGAG AAGGGAATGG CTGGAGGACA TGGTCCTGAT
2221     GGCCCAAAAG GCAGTCCAGG TCCATCTGGG ACCCCTGGAG ATACAGGCCC ACCAGGTCTT
2281     CAAGGTATGC CGGGAGAAAG AGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT
2341     GGCATAGGAG AAAAAGGTGC TGAAGGCACA GCTGGAAATG ATGGTGCAAG AGGTCTTCCA
2401     GGTCCTTTGG GCCCTCCAGG TCCGGCAGGT CCTACTGGAG AAAAGGGTGA ACCTGGTCCT
2461     CGAGGTTTAG TTGGCCCTCC TGGCTCCCGG GGCAATCCTG GTTCTCGAGG TGAAAATGGG
2521     CCAACTGGAG CTGTTGGTTT TGCCGGACCC CAGGGTCCTG ACGGACAGCC TGGAGTAAAA
2581     GGTGAACCTG GAGAGCCAGG ACAGAAGGGA GATGCTGGTT CTCCTGGACC ACAAGGTTTA
2641     GCAGGATCCC CTGGCCCTCA TGGTCCTAAT GGTGTTCCTG GACTAAAAGG TGGTCGAGGA
2701     ACCCAAGGTC CGCCTGGTGC TACAGGATTT CCTGGTTCTG CGGGCAGAGT TGGACCTCCA
2761     GGCCCTGCTG GAGCTCCAGG ACCTGCGGGA CCCCTAGGGG AACCCGGGAA GGAGGGACCT
2821     CCAGGTCTTC GTGGGGACCC TGGCTCTCAT GGGCGTGTGG GAGATCGAGG ACCAGCTGGC
2881     CCCCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGGCAACC TGGTCCAGAT
2941     GGCCCCCCTG GTCCAGCTGG AACGACCGGG CAGAGAGGAA TTGTTGGCAT GCCTGGGCAA
3001     CGTGGAGAGA GAGGCATGCC CGGCCTACCA GGCCCAGCGG GAACACCAGG AAAAGTAGGA
3061     CCAACTGGTG CAACAGGAGA TAAAGGTCCA CCTGGACCTG TGGGGCCCCC AGGCTCCAAT
3121     GGTCCTGTAG GGGAACCTGG ACCAGAAGGT CCAGCTGGCA ATGATGGTAC CCCAGGACGG
3181     GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC
3241     TCTCAGGGTG CCCCTGGAAC TCCTGGCCCT GTGGGTGCTC CAGGAGATGC AGGACAAAGA
3301     GGAGATCCGG GTTCTCGGGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGATTA
3361     CCTGGACCCC AAGGACCTCG TGGTGACAAA GGTGATCATG GAGACCGAGG CGACAGAGGT
3421     CAGAAGGGCC ACAGAGGCTT TACTGGTCTT CAGGGTCTTC CTGGCCCTCC TGGTCCAAAT
3481     GGTGAACAAG GAAGTGCTGG AATCCCTGGA CCATTTGGCC CAAGAGGTCC TCCAGGCCCA
3541     GTTGGTCCTT CAGGTAAAGA AGGAAACCCT GGGCCACTTG GGCCAATTGG ACCTCCAGGT
3601     GTACGAGGCA GTGTAGGAGA AGCAGGACCT GAGGGCCCTC CTGGTGAGCC TGGCCCACCT
3661     GGCCCTCCGG GTCCCCCTGG CCACCTTACA GCTGCTCTTG GGGATATCAT GGGGCACTAT
3721     GATGAAAGCA TGCCAGATCC ACTTCCTGAG TTTACTGAAG ATCAGGCGGC TCCTGATGAC
3781     AAAAACAAAA CGGACCCAGG GGTTCATGCT ACCCTGAAGT CACTCAGTAG TCAGATTGAA
3841     ACCATGCGCA GCCCCGATGG CTCGAAAAAG CACCCAGCCC GCACGTGTGA TGACCTAAAG
3901     CTTTGCCATT CCGCAAAGCA GAGTGGTGAA TACTGGATTG ATCCTAACCA AGGATCTGTT
3961     GAAGATGCAA TCAAAGTTTA CTGCAACATG GAAACAGGAG AAACATGTAT TTCAGCAAAC
4021     CCATCCAGTG TACCACGTAA AACCTGGTGG GCCAGTAAAT CTCCTGACAA TAAACCTGTT
4081     TGGTATGGTC TTGATATGAA CAGAGGGTCT CAGTTCGCTT ATGGAGACCA CCAATCACCT
4141     AATACAGCCA TTACTCAGAT GACTTTTTTG CGCCTTTTAT CAAAAGAAGC CTCCCAGAAC
4201     ATCACTTACA TCTGTAAAAA CAGTGTAGGA TACATGGACG ATCAAGCTAA GAACCTCAAA
4261     AAAGCTGTGG TTCTCAAAGG GGCAAATGAC TTAGATATCA AAGCAGAGGG AAATATTAGA
4321     TTCCGGTATA TCGTTCTTCA AGACACTTGC TCTAAGCGGA ATGGAAATGT GGGCAAGACT
4381     GTCTTTGAAT ATAGAACACA GAATGTGGCA CGCTTGCCCA TCATAGATCT TGCTCCTGTG
4441     GATGTTGGCG GCACAGACCA GGAATTCGGC GTTGAAATTG GGCCAGTTTG TTTTGTGTAA

Quality Control:cDNA sequencing results is available upon request.