Human COL21A1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13407-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

COL21A1 cDNA Clone Datasheet

COL21A1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_030820.3
cDNA Size:	2874
Gene Synonym:	FP633; COLA1L; dJ708F5.1; dJ682J15.1; COL21A1
Source:	Human

COL21A1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens collagen, type XXI, alpha 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1355 G/A resulting in the amino acid Gly substitution by Glu and 90 T/C，438 A/G not causing the amino acid variation.

COL21A1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

COL21A1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

COL21A1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCACT ATATTACATT TCTCTGCATG GTTTTGGTGC TGCTTCTTCA GAATTCTGTG
61       TTAGCTGAAG ATGGGGAAGT AAGATCAAGC TGTCGTACTG CTCCGACAGA TTTAGTTTTC
121      ATCTTAGATG GCTCTTATAG TGTTGGCCCA GAAAACTTTG AAATAGTGAA AAAGTGGCTT
181      GTCAATATCA CAAAAAACTT TGACATAGGG CCGAAGTTTA TTCAAGTTGG AGTGGTTCAA
241      TATAGTGACT ACCCTGTGCT GGAGATTCCT CTCGGAAGCT ATGATTCAGG AGAACATTTG
301      ACGGCAGCAG TGGAATCCAT ACTCTACTTA GGAGGAAACA CAAAGACAGG GAAGGCCATC
361      CAGTTTGCGC TCGATTACCT TTTTGCCAAG TCCTCACGAT TTCTGACTAA GATAGCAGTG
421      GTACTTACGG ATGGCAAGTC CCAAGATGAC GTCAAGGATG CAGCTCAAGC AGCAAGAGAT
481      AGTAAGATAA CATTATTTGC TATTGGTGTT GGTTCAGAAA CAGAAGATGC CGAACTTAGA
541      GCTATTGCCA ACAAGCCTTC GTCTACTTAT GTGTTTTATG TGGAAGACTA TATTGCAATA
601      TCCAAAATAA GGGAAGTGAT GAAGCAGAAA CTTTGTGAAG AATCTGTCTG TCCAACACGA
661      ATTCCAGTGG CAGCTCGTGA TGAAAGGGGA TTTGATATTC TTTTAGGTTT AGATGTAAAT
721      AAAAAGGTTA AGAAAAGAAT ACAGCTTTCA CCAAAAAAGA TAAAAGGATA TGAAGTAACA
781      TCAAAAGTTG ATTTATCAGA ACTCACAAGC AATGTTTTCC CAGAAGGTCT TCCTCCATCA
841      TATGTATTTG TGTCTACTCA AAGATTTAAA GTCAAGAAAA TTTGGGATTT ATGGAGAATA
901      TTAACTATTG ATGGAAGGCC ACAAATAGCA GTTACCTTAA ATGGTGTGGA CAAAATCTTA
961      TTATTTACAA CAACCAGCGT AATTAATGGC TCACAAGTGG TTACCTTTGC TAACCCTCAA
1021     GTTAAGACGT TGTTTGATGA AGGCTGGCAC CAAATTCGTC TCTTAGTAAC AGAACAAGAT
1081     GTGACTTTGT ATATTGATGA CCAACAAATT GAAAACAAGC CCTTACATCC AGTTTTAGGG
1141     ATCTTGATCA ATGGGCAAAC CCAAATTGGA AAATATTCTG GAAAAGAAGA AACTGTTCAG
1201     TTTGATGTCC AAAAGTTGCG AATCTACTGT GACCCAGAAC AGAACAACCG GGAGACAGCA
1261     TGTGAGATTC CTGGATTTAA TGGAGAGTGC CTTAATGGTC CCAGTGATGT AGGTTCAACT
1321     CCAGCTCCCT GTATTTGTCC TCCGGGAAAA CCAGAACTTC AAGGCCCCAA AGGTGACCCT
1381     GGACTGCCTG GGAACCCTGG CTACCCTGGA CAACCTGGTC AAGATGGTAA GCCTGGATAT
1441     CAGGGAATTG CAGGGACACC AGGTGTTCCA GGATCTCCAG GAATACAAGG AGCTCGAGGA
1501     CTACCAGGTT ACAAAGGAGA ACCAGGGCGA GATGGTGACA AGGGTGATCG TGGACTTCCT
1561     GGTTTTCCTG GGCTTCATGG CATGCCAGGA TCAAAGGGTG AAATGGGTGC CAAAGGAGAC
1621     AAAGGATCAC CTGGATTTTA TGGCAAAAAG GGTGCAAAAG GTGAAAAGGG GAATGCTGGC
1681     TTCCCTGGCC TCCCTGGACC TGCTGGAGAA CCAGGAAGAC ATGGAAAGGA TGGATTAATG
1741     GGTAGTCCCG GTTTCAAGGG AGAAGCAGGA TCCCCTGGTG CTCCGGGGCA GGATGGAACA
1801     CGGGGAGAGC CTGGAATCCC AGGATTTCCT GGAAACCGAG GATTAATGGG CCAAAAGGGA
1861     GAAATTGGGC CTCCAGGACA GCAAGGAAAA AAAGGAGCCC CAGGGATGCC TGGTTTAATG
1921     GGAAGCAATG GCTCACCAGG CCAGCCTGGA ACACCGGGAT CTAAGGGAAG CAAAGGTGAA
1981     CCTGGAATTC AAGGGATGCC TGGGGCTTCT GGGCTCAAGG GAGAACCAGG AGCAACGGGT
2041     TCCCCAGGAG AACCAGGATA CATGGGTTTA CCCGGGATTC AAGGAAAAAA GGGGGACAAA
2101     GGAAATCAAG GTGAAAAAGG TATTCAGGGT CAAAAGGGAG AAAATGGAAG ACAGGGAATT
2161     CCAGGGCAAC AGGGAATTCA AGGCCATCAT GGTGCAAAAG GAGAGAGAGG TGAAAAGGGA
2221     GAACCTGGTG TCCGAGGTGC CATTGGATCA AAAGGAGAAT CTGGGGTGGA TGGCTTGATG
2281     GGGCCCGCAG GTCCTAAGGG GCAACCTGGG GATCCAGGTC CTCAGGGACC CCCAGGTTTG
2341     GATGGGAAGC CCGGAAGAGA GTTTTCAGAA CAATTTATTC GACAAGTTTG CACAGATGTA
2401     ATAAGAGCCC AGCTACCAGT CTTACTTCAG AGTGGAAGAA TTAGAAATTG TGATCATTGC
2461     CTGTCCCAAC ATGGCTCCCC GGGTATTCCT GGGCCACCTG GTCCGATAGG CCCAGAGGGT
2521     CCCAGAGGAT TACCTGGTTT GCCAGGAAGA GATGGTGTTC CTGGATTAGT GGGTGTCCCT
2581     GGACGTCCAG GTGTCAGAGG ATTAAAAGGC CTACCAGGAA GAAATGGGGA AAAAGGGAGC
2641     CAAGGGTTTG GGTATCCTGG AGAACAAGGT CCTCCTGGTC CCCCAGGTCC AGAGGGCCCT
2701     CCTGGAATAA GCAAAGAAGG TCCTCCAGGA GACCCAGGTC TCCCTGGCAA AGATGGAGAC
2761     CATGGAAAAC CTGGAATCCA AGGGCAACCA GGCCCCCCAG GCATCTGCGA CCCATCACTA
2821     TGTTTTAGTG TAATTGCCAG AAGAGATCCG TTCAGAAAAG GACCAAACTA TTAG

Quality Control:cDNA sequencing results is available upon request.