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Human COL21A1 Gene cDNA Clone (full-length ORF Clone)

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    Human COL21A1 cDNA Clone Product Information
    NCBI RefSeq:NM_030820.3
    RefSeq ORF Size:2874
    cDNA Description:ORF Clone of Homo sapiens collagen, type XXI, alpha 1 DNA.
    Gene Synonym:FP633, COLA1L, dJ708F5.1, dJ682J15.1, COL21A1
    Species:Human
    Vector:pGEM-T Vector
    Plasmid:
    Restriction Site:
    Tag Sequence:
    Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1355 G/A resulting in the amino acid Gly substitution by Glu and 90 T/C,438 A/G not causing the amino acid variation.
    Sequencing primers:
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:
    Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
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