Rat CNTN5 Gene cDNA Clone / ORF Clone

CNTN5 cDNA Clone Datasheet

CNTN5 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_053746.1
• cDNA Size: 3300
• Gene Synonym: Nb-2; Cntn5
• Source: Rat

CNTN5 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus contactin 5 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 314 C/A，resulting in the amino acid Pro substitution by His and 1161 C/T，2946 T/C not causing the amino acid variation.

CNTN5 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CNTN5 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CNTN5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTTCCT CTTGGAGACT AATCCTGTTC CTGTCTTTCA CAAGTTGTCT TTCAGAATAC
61       TCTGAAGCTC TCTCTGGCCT TTCTACTTCA TATGCTGCTT TGCTAAGAAT TAAGAAGAGT
121      TCAACCTCAT CTGCCTTCGG TTCCAAGTCC AGACCACGAT ACAGCAGTCC TTCATTAGGC
181      ACCCTCAGTG TTTCTCCACC GAGCTGGCGA GGGGCAGCTC AGCAGTACCA CTCCCCGGTT
241      AACCTTTACC ACTCCCCAGA TGCCTTCAGA CAGGATGAAA GTGTGGATTA TGGGCCAGTA
301      TTTGTGCAAG AGCATGATGA TATTATTTTT CCAACTGATT CTGATGAAAA GAAGGTAGCC
361      CTGAATTGTG AAGTTCGTGG CAACCCATCT CCTACTTACA GATGGCTTCG GAATGGAACA
421      GAAATTGATC TTGAGAGTGA CTACCGCTAC AGTATGATAG ACGGCACCTT CATCATCAAC
481      AATCCAAGTG AAAGCAGGGA TTCAGGGCTC TACCAGTGTT TAGCCACCAA CACGTTTGGA
541      AGTATTCTCA GTCGAGAAGC CACACTTCAG TTTGCTTATC TGGGGAATTT TAGTGGCCGG
601      ACAAGAAGTG CAGTCTCAGT GCGGGAAGGC CAGGGGGTTG TTCTGATGTG CTCTCCACCG
661      CCTCATTCTC CAGAGATCAT CTATAGCTGG GTCTTCAATG AGTTCCCTTC CTTCGTGGCA
721      GAGGACAGCC GGCGGTTCAT ATCCCAAGAG ACAGGCAACC TCTATATTTC TAAAGTGCAA
781      ACATCAGATG TTGGCAGCTA CATTTGTCTG GTGAAAAATG CTGTAACGAA TGCTCGCGTG
841      CTCAGCCCTC CAACCCCGCT CACGCTTCGA AATGATGGTG TAATGGGAGA ATACGAGCCA
901      AAAATTGAGG TCCATTTTCC CACCACGGTC ACAGCTGCTA AGGGAACGAC TGTGAAGATG
961      GAATGCTTTG CACTTGGCAA CCCTGTTCCA ACGATCACTT GGATGAAGGT TAACGGTTAC
1021     ATTCCCAGTA AGTCCAGACT GCGAAAATCC CAGGCGGTCC TGGAGATCCC CAACCTGCAG
1081     CTGGATGACG CCGGAATATA TGAATGCACA GCTGAAAACT CCCGAGGGAA AAACTCCTTT
1141     CGTGGACAGC TCCAAATATA TACGTACCCA CACTGGGTAC AGAAACTGAA TGACACCCAG
1201     CTGGACAGTG GGAGTCCTCT GCAGTGGGAA TGCAAGGCTA CCGGAAAGCC CAGGCCCACA
1261     TACCGCTGGC TGAAGAATGG AGCACCCCTC CTGCCACAGA GCAGAGTGGA CACAGCTAAT
1321     GGAGTATTGG CGATCCATAG TGTAAATCAA TCAGATGCTG GGATGTATCA GTGTTTGGCT
1381     GAGAATAAAT ATGGAGCTAT TTATGCGAGC GCCGAGCTGA AGATTTTAGC CTCACCACCC
1441     TCTTTTGAAC TGAATCAAGT GAAGAAATCA ATAATTGTCA CGAAAGACCG GGAAGTGCTC
1501     ATAGAATGCA AACCCCAGGG CTCTCCAAAG CCAGCCATCT CCTGGAGGAA GGGAGACAAG
1561     GCAGTGAGGG GGAACAAGAG AATAGCTATT CTTCCAGACG GAAGCCTGCG GATCCTCAAT
1621     GCTTCTAAAG CGGATGAGGG GAAGTACATT TGCCAAGGGG TGAACATCTT CGGTTCCGCG
1681     GAGATCATAG CTTCTGTGTC TGTCAAAGAA CCCACAAGGA TAGAGCTTAC TCCTAAAAGA
1741     ACAGAGTTAA CTGTTGGAGA GAGCATTGTC CTTAACTGCA AAGCCATGCA CGACTCCAGT
1801     TTGGATGTCA CTTTCTACTG GACACTAAAA GGACAACCTA TTGATTTTGA GAAAGAAGGC
1861     GGACATTTTG AAAGCATCAG GGCTCAAGCA TCCTCTGCAG ATTTAATGAT CAGGAACATC
1921     CTTCTGATGC ATGCTGGGAG ATATGGCTGC AGGGTACAGA CCACAGCAGA CAGTGTGTCA
1981     GATGAGGCAG AACTTCTTGT TAGGGGTCCC CCAGGGCCAC CTGGAGTGGT CATAGTGGAG
2041     GAGATCACAG AAAGCACAGC CACTCTCTCA TGGAGTCCAG CAACTGACAA CCACAGCCCC
2101     ATCTCTTCCT ACAACCTTCA GGCTCGCAGC CCCTTCTCCC TGGGCTGGCA AACAGTGAAG
2161     ACAGTCCCTG AAGTCATCAC AGGAGACATG GAGTCAGCGA TGGCTGTGGA CCTAAACCCC
2221     TGGGTAGAAT ATGAATTCCG AGTGGTGGCC ACCAATCCAA TTGGGACAGG AGATCCAAGC
2281     ATTCCATCTC GAATGATCCG CACAAACGAG GCAGTTCCTA AGACGGCACC CAGCAACGTG
2341     AGTGGAGGAA GTGGAAGAAG GCACGAGTTG GTCATTGCCT GGGAGCCAGT ATCAGAAGAG
2401     TTTCAGAATG GGGAAGGCTT CGGCTACATA GTGGCATTCA GACCGAATGG AACACGTGGC
2461     TGGAAGGAGA AAATGGTTAC TTCTTCGGAT GCTTCCAAGT TCATTTACCG AGACGAAAGT
2521     GTCCCTCCTC TCACACCCTT TGAAGTCAAA GTTGGTGTCT ATAATAATAA AGGTGATGGG
2581     CCTTTCAGCC AGATTGTAGT CATCTGCTCG GCTGAGGGAG AACCCACTGC TGCTCCCACT
2641     GATGTCACAG CGACCAGTGT GTCGGTGTCA GAGATTTTTG TTGTATGGAA ACATGTTAAA
2701     GAGAGTCTCG GAAGACCACA GGGGTTTGAG ATTGGTTACT GGAAAGATAC CGAGCCAGAA
2761     GACTCTGCAG AAACAGTCAG AACCAGAGGG AATGAGTCAT TCGTCATGTT GACGGGATTG
2821     GAAGGCGACA CACTCTATCA CCTCACAGTC AGGGCTTACA ATGGAGCCGG GTATGGGCCA
2881     CCCAGCAGGG AAGTGAGCGC CACCACCAAG AGGCACCCCC CTAGCGAACC ACCTGGCAAC
2941     CTCCGCTGGG AACAGCAGGG TTCCCAGGTT TCCCTAGGCT GGGAGCCTGT CAGACCTCTA
3001     GCCAACGAGT CTGAAGTCAT GGGTTACAAG GTTTTTTATA GGCAAGAGGG TCACAGCAAA
3061     GGTCAAGTTA TTGAGACACA GAAACCTCAG GCTGTTGTGC CTCTCCCTGA GGCTGGAGTC
3121     TACATTATTG AAGTTCGAGC GTACAGTGAA GGAGGAGATG GAACAGCGAG CTCCCAAATC
3181     AGGGTACCTT CATATGCAGG TGGGAAAATC ACAAGCGCTC AGTCCACCCT GCACTCTCTC
3241     TCCAAGTGGT CCTCAGTAAC ACTGCTCTTG GCTTTGATGC TTCCTTCCAG TTCCTGGTGA

 

Quality Control:cDNA sequencing results is available upon request.