Mouse CNTN5 Gene cDNA Clone / ORF Clone

CNTN5 cDNA Clone Datasheet

CNTN5 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001033359.2
• cDNA Size: 2682
• Gene Synonym: NB-2; Gm507; 6720426O10Rik; A830025P08Rik; Cntn5
• Source: Mouse

CNTN5 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus contactin 5 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1072 G/A resulting in the amino acid Ala substitution by Thr and 948 G/T not causing the amino acid variation.

CNTN5 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CNTN5 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CNTN5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTTCCT GTTGGAGACT CATTCTGTTC CTGTCTGTCA CCAGGTGGCT TTCAGAGATC
61       ATCTATAGCT GGGTCTTCAA TGAGTTCCCT TCCTTTGTGG CAGAGGACAG CCGGCGGTTC
121      ATATCCCAAG AGACAGGCAA CCTTTATATT TCTAAAGTGC AAACATCCGA TGTTGGCAGC
181      TACATTTGTC TGGTGAAAAA TGCTGTGACA AATGCTCGCG TGCTCAGTCC TCCAACCCCA
241      CTCACGCTGC GAAATGATGG TGTGATGGGA GAATATGAGC CAAAAATTGA GGTCCATTTT
301      CCTTTCACTG TCACAGCTGC TAAGGGAACA ACTGTGAAGA TGGAATGCTT TGCACTTGGC
361      AACCCGGTTC CAACAATTAC TTGGATGAAG GTTAACGGTT ACATTCCTAG TAAGTCCAGA
421      CTGCGGAAAT CCCAGGCAGT GCTGGAGATT CCAAACCTGC AGCTGGATGA TGCCGGAATA
481      TACGAATGCA CAGCTGAAAA CTCCCGTGGA AAAAACTCCT TTCGTGGACA GCTACAAATA
541      TTCACATACC CACACTGGGT ACAGAAACTG AATGATACCC AGCTGGACAG TGGGAGTCCT
601      CTGCAGTGGG AATGCAAGGC TACTGGAAAG CCCAGACCCA CATACCGCTG GCTGAAGAAT
661      GGAGCACCCC TCTTGCCGCA GAGCAGAGTG GACACAGTGA ATGGAATATT GGCGATCCAG
721      AGTGTGAATC AATCAGATGC TGGGATGTAT CAGTGTTTGG CTGAGAATAA ATATGGAGCT
781      ATTTATGCGA GTGCTGAGCT GAAGATTCTA GCTTCGCCGC CCTCCTTCGA ACTGAATCAA
841      GTGAAGAAAT CCATAATTGT TACCAAAGAC CGGGGAGTGC TCATAGAGTG CGAACCCCAG
901      GGATCTCCAA AGCCAGCCAT CTCCTGGAGG AAGGGAGACA AGGCAGTTAG GGCGAATAAA
961      AGAATAGCTA TTCTTCCAGA CGGAAGCCTG AGGATCCTAA ACGCTTCTAA AGCAGATGAG
1021     GGGAAGTACA TTTGCCAAGG GGTGAACATC TTCGGTTCTG CAGAGATTAT AACTTCCCTG
1081     TCTGTCAAAG AACCCACAAG GATAGAGCTT ACTCCTAAAA GAACAGAGTT AACTGTTGGA
1141     GAGAGCATTG TCCTCAACTG TAAAGCTATC CACGACGCCA GTTTGGACGT CACTTTCTAC
1201     TGGACATTAA AAGGACAACC TATTGATTTT GAGAAAGAAG GCGGACATTT TGAAAACATC
1261     AGGGCTCAAG CATCCTCTGC AGATTTAATG ATCAGGAACA TCCTTCTGAT GCATGCTGGG
1321     AGATATGGCT GCAGGGTACA GACCACAGCA GACAGTGTGT CAGATGAGGC AGAACTTCTT
1381     GTTAGGGGTC CCCCAGGGCC ACCTGGAGTG GTCATAGTAG AGGAGATTAC AGAAAGCACA
1441     GCCACTCTCT CATGGAGTCC AGCAACTGAC AACCATAGCC CCATCTCTTC TTACAACCTT
1501     CAGGCTCGCA GCCCCTTCTC CCTGGGCTGG CAAACAGTGA AGACAGTCCC AGAAGTCATC
1561     ACAGGAGACA TGGAGTCAGC AATGGCTGTG GACCTAAACC CCTGGGTAGA ATATGAATTC
1621     CGAGTGGTGG CCACCAATCC AATTGGGACA GGAGATCCAA GCATTCCATC TCGAATGATC
1681     CGCACAAATG AGGCAGTTCC AAAGACGGCA CCCAGCAACG TGAGTGGAAG AAGTGGAAGA
1741     AGGCATGAGT TGGTCATTGC CTGGGAGCCA GTATCAGAAG AGTTTCAGAA TGGGGAAGGC
1801     TTCGGCTACA TAGTGGCATT CAGACCTAAT GGAACACGTG GCTGGAAGGA GAAAATGGTT
1861     ACGTCTTCGG AGGCGTCCAA GTTCATTTAC CGAGATGAAA GTGTCCCTCC TCTCACACCC
1921     TTTGAGGTCA AAGTTGGTGT TTATAATAAT AAAGGTGATG GGCCTTTCAG CCAGATCGTA
1981     GTCATCTGTT CAGCTGAGGG AGAACCCACT GCTGCTCCCA CTGACGTCAC AGCAACCAGT
2041     GTGTCAGTGT CAGAGATTTT TGTTGTATGG AAACATGTTA AGGAGAGTCT TGGAAGACCA
2101     CAGGGGTTTG AGATTAGTTA CTGGAAAGAT ACCGAGCCAG AAGACTCCGT AGAAACAGTC
2161     CGAACCAGAG GGAATGAGTC ATTCGTCATG TTGACGGGAT TGGAAGGCAA CACACTCTAT
2221     CACCTCACAG TCAGGGCTTA CAATGGAGCC GGCTATGGGC CACCCAGCAG GGAAGCAAGC
2281     ACCACCACAA AGAGGCACCC CCCTAGGGAG CCACCTGGCA ACCTCCGGTG GGAGCAGCAG
2341     GGCTCCCAAG TTTCCCTAGG CTGGGAGCCA GTCAGACCCC TAGCCAATGA GTCTGAAGTC
2401     ATGGGTTACA AGGTTTTTTA TAGGCAAGAG GGTCACAGCG AAGGTCAAGT TATTGAGACA
2461     CAGAAACCTC AAGCTGTAGT GCCTCTACCT GAGGCTGGAG TCTACATTAT TGAAGTTCGA
2521     GCGTACAGTG AGGGAGGAGA TGGAACAGCT AGCTCCCAAA TCAGGGTTCC TTCATATTCA
2581     GGTGGGAAAA TCACAAGCGC TCAGTCAACC CTGCACTCTC TCTCCAAGTG GTCCTCAGTA
2641     ACACTGCTCT TGGCTTTGAT GCTTCCTTCC AGTTCCTGGT GA

 

Quality Control:cDNA sequencing results is available upon request.