Rat CNTN3 Gene cDNA Clone / ORF Clone

CNTN3 cDNA Clone Datasheet

CNTN3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_019329.1
• cDNA Size: 3087
• Gene Synonym: Pang; BIG-1; Cntn3
• Source: Rat

CNTN3 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus contactin 3 (plasmacytoma associated) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 2310 T/C and 2385 G/A not causing the amino acid variation.

CNTN3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CNTN3 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CNTN3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATGTTGT CATGGAAACA GTTGATCCTG CTTTCATTCA TTGGCTGCCT GGCAGGTGAG
61       CTTCTTTTGC AAGGCCCCGT GTTTGTCAAA GAGCCCAGCA ACAGTATTTT CCCTGTTGGC
121      TCGGAGGATA AAAAAATAAC ATTAAATTGT GAAGCAAGAG GCAACCCTTC TCCTCATTAC
181      AGGTGGCAGC TGAATGGCAG TGACATTGAT ACAAGTCTGG ACCATCGTTA CAAACTGAAC
241      GGAGGAAATC TCATAGTTAT TAACCCTAAC AGAAATTGGG ATACTGGAAG TTACCAGTGT
301      TTTGCAACAA ATTCTCTTGG AACAATAGTC AGCAGAGAAG CTAAACTCCA ATTTGCCTAT
361      CTTGAAAATT TTAAGAGCAG AATGAGAAGC AGAGTGTCAG TTCGTGAAGG CCAGGGAGTG
421      GTACTTCTCT GTGGACCCCC ACCACACTCC GGGGAACTTT CATATGCTTG GGTCTTCAAT
481      GAGTATCCAT CATTCGTTGA AGAAGACAGT CGAAGATTTG TCTCTCAAGA GACAGGACAC
541      CTCTACATAG CTAAAGTGGA GCCATCTGAT GTGGGAAATT ACACATGCGT GGTGACCAGC
601      ACTGTGACAA ATGCCAGAGT ATTGGGCTCC CCAACCCCTT TGGTGCTACG CTCTGATGGT
661      GTCATGGGTG AATATGAACC TAAAATAGAA CTCCAGTTTC CAGAAACTCT TCCAGCAGCT
721      AAAGGTTCAA CTGTGAAGTT GGAATGCTTC GCCCTAGGGA ACCCTGTTCC TCAGATTAAT
781      TGGAGAAGAA GTGATGGGAT GCCGTTTCCC ACCAAAATTA AGCTGAGGAA GTTCAATGGT
841      GTGCTCGAAA TTCCAAACTT CCAACAGGAA GATACAGGCT CCTATGAGTG CATTGCTGAA
901      AATTCACGAG GAAAGAATGT GGCGAGAGGA CGGCTCACTT ACTATGCAAA ACCTTATTGG
961      GTTCAACTCT TAAAAGATGT GGAAACTGCT GTGGAGGACA GTCTTTATTG GGAATGCCGG
1021     GCAAGTGGGA AGCCTAAACC CTCCTATCGA TGGCTGAAAA ATGGAGATGC CTTAGTACTA
1081     GAGGAGAGAA TACAGATAGA AAATGGTGCC CTTACAATAG CCAACCTCAA TGTGAGTGAC
1141     TCTGGAATGT TCCAGTGTAT AGCAGAGAAC AAGCATGGCC TCATCTACTC CAGTGCTGAG
1201     CTCAAAGTAT TAGCTTCTGC TCCAGATTTT TCAAGGAACC CTATGAAAAA AATGATTCAG
1261     GTGCAGGTGG GCAGCCTGGT GATCTTGGAT TGTAAACCCA GCGCCTCTCC AAGGGCACTT
1321     TCTTTCTGGA AGAAGGGAGA CACGGTGGTG CGGGAGCAGG CAAGGATTTC TCTTTTAAAT
1381     GATGGAGGAC TCAAAATTAT GAACGTGACC AAGGCTGATG CTGGAATCTA CACCTGCATA
1441     GCAGAAAACC AGTTTGGCAA AGCGAATGGC ACCACCCAGT TGGTTGTGAC AGAACCAACA
1501     AGAATAATTT TGGCACCTTC TAACATGGAT GTTGCCGTTG GTGAGAGCAT CATTTTACCT
1561     TGCCAGGTTC AACATGACCC CCTATTAGAC ATAATGTTTG CCTGGTATTT CAATGGAACT
1621     CTTACAGACT TTAAGAAAGA TGGATCGCAC TTTGAGAAAG TTGGTGGGAG TTCGTCAGGT
1681     GATTTAATGA TCAGAAACAT TCAACTGAAA CACAGTGGAA AGTATGTATG TATGGTGCAG
1741     ACAGGGGTGG ACAGCGTTTC ATCTGCAGCT GAGCTCATAG TGAGAGGTTC ACCTGGACCA
1801     CCGGAAAACG TGAAAGTAGA TGAAATTACA GACACAACAG CCCAGCTCTC TTGGACGGAA
1861     GGCACCGACA GTCACAGCCC AGTTATATCG TATGCAGTCC AGGCTAGGAC ACCTTTCTCT
1921     GTGGGCTGGC AAAATGTCAG AACAGTACCT GAGGCCATTG ATGGAAAAAC ACGAACAGCT
1981     ACTGTTGTGG AGTTAAACCC ATGGGTGGAA TATGAATTTC GCGTTGTAGC CAGTAACAAA
2041     ATCGGAGGCG GAGAACCCAG TTTACCCTCA GAAAAAGTAA GGACTGAAGA GGCAGCTCCA
2101     GAAGTCGCAC CTTCTGAGGT GAGCGGAGGA GGTGGAAGCC GATCTGAGCT AGTGATAACC
2161     TGGGATCCAG TCCCTGAAGA ACTCCAGAAT GGAGGAGGTT TTGGGTATGT GGTTGCTTTT
2221     CGCCCACTTG GGGTTACCAC CTGGATCCAG ACAGTGGTGA CATCCCCAGA TAATCCAAGA
2281     TACGTCTTTA GGAATGAAAG CATCGTGCCC TTTTCGCCAT ATGAAGTTAA AGTGGGCGTT
2341     TACAATAACA AAGGTGAAGG ACCCTTTAGC CCAGTCACAA CTGTATTCTC CGCAGAGGAA
2401     GAGCCTACAG TCGCCCCATC TCACATCTCT GCACATAGTC TGTCCTCCTC AGAAATTGAA
2461     GTTTCATGGA ACACAATTCC CTGGAAGTCG AGCAATGGAC GTTTACTTGG CTATGAGGTG
2521     CGGTACTGGA ACAATGGAGG AGAAGAAGAA TCATCCAGCA AAGTGAAAGT GGCAGGAAAT
2581     CAGACATCAG CTGTGCTCCG AGGCCTGAAA AGCAACCTGG CATATTACAC AGCCGTCCGG
2641     GCTTACAACA CCGCAGGCGC TGGTCCCTTC AGTGCCACAG TTAACGCGAC CACCAAGAAA
2701     ACCCCTCCCA GTCAGCCACC AGGAAATGTT GTTTGGAATG CTACAGACAC TAAAGTGCTG
2761     CTTAACTGGG AACAAGTGAA AGCCTTGGAG AATGAATCAG AAGTAACAGG CTACAAGGTT
2821     TTCTATAGGA CTAGCAGTCA AAATAATGTG CAAGTACTGA ACACAAACAA AACTTCAGCT
2881     GAACTGTTGC TGCCTATCAA GGAAGACTAC ATCATTGAAG TCAAGGCCAC CACAGATGGA
2941     GGGGACGGCA CCAGTAGTGA GCAGATCAGG ATTCCAAGAA TAACCAGTAT GGATGCAAGA
3001     GGATCCACTT CAGCCATTTC TGACATCCAC CCTGTGTCAG GTTACATATC CGTCCTACTG
3061     TTCTTTATTG TAAATGCTTT GTGGTGA

 

Quality Control:cDNA sequencing results is available upon request.