Rat CNTN3 / Contactin-3 Protein

Contactin 3 (plasmacytoma associated) Protein Datasheet

CNTN3 / Contactin-3 Protein Product Information

• Synonym: CNTN3
• Protein Construction: A DNA sequence encoding the rat CNTN3 (Met1-Gly1001) was expressed with the Fc region of human IgG1 at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

CNTN3 / Contactin-3 Protein QC Testing

• Purity: > 85% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 20
• Molecular Mass: The recombinant rat CNTN3/Fc is a disulfide-linked homodimer. The reduced monomer comprises 1224 amino acids and has a predicted molecular mass of 134.8 kDa. The apparent molecular mass of the protein is approximately 135 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CNTN3 / Contactin-3 protein

CNTN3 / Contactin-3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CNTN3 / Contactin-3 Protein Description

The brain-derived axon-associated cell adhesion molecule BIG-1, also known as CNTN3 or PANG (plasmacytoma-associated neuronal glycoprotein) ,belongs to the contactin family consisting of six members (CNTN1/F3, CNTN2/TAG-1, CNTN3/BIG-1, CNTN4/BIG-2, CNTN5/NB-2 and CNTN6/NB-3). CNTN3 is a member of the immunoglobulin (Ig)superfamily with six Ig-like domains, four fibronectin type I II-like repeats, and a GPI-anchoring domain. The expression pattern of CNTN3 is developmentally regulated with the highest level in the adult brain , and restricted to subsets of neurons such as Purkinje cells of the cerebellum, granule cells of the dentate gyrus, and neurons in the superficial layers of the cerebral cortex. It has been suggested that BIG-1 has a neurite outgrowth-promoting activity ,and play an important role in the formation and maintenance of specific neuronal networks in the brain together with other contactin members.

References

1. Yoshihara Y. et al., 1994, Neuron. 13: 415-26.
2. Yoshihara Y. et al., 1995, J Neurobiol. 28: 51-69.
3. Connelly M.A., et al., 1994, Proc Natl Acad Sci. 91: 1337-41.
4. Mock BA. et al., 1996, Genomics. 34: 226-8.