Rat CNTN2 Gene cDNA Clone / ORF Clone

CNTN2 cDNA Clone Datasheet

CNTN2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_012884.1
• cDNA Size: 3123
• Gene Synonym: Tax; TAG1; TAG-1; TAG-564; Cntn2
• Source: Rat

CNTN2 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus contactin 2 (axonal) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 558 C/T not causing the amino acid variation.

CNTN2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CNTN2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CNTN2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGACAC ACGCCAGGAA AAAGGCAAGC TTGCTGCTGC TGGTGCTGGC CACAGTGGCC
61       CTGGTCTCCT CTCCAGGATG GAGTTTTGCC CAGGGAACCC CAGCTACCTT TGGACCCATC
121      TTCGAAGAGC AACCCATTGG CCTGCTATTC CCAGAGGAGT CTGCAGAGGA TCAGGTGACA
181      CTGGCGTGCC GTGCCCGTGC TAGCCCTCCA GCCACCTACA GGTGGAAGAT GAATGGCACA
241      GATATGAACC TGGAACCTGG CTCCCGTCAC CAGCTGATGG GGGGCAACCT GGTCATCATG
301      AGCCCCACCA AGACACAGGA TGCTGGTGTC TACCAGTGCC TAGCCTCCAA CCCAGTAGGC
361      ACTGTGGTCA GCAAGGAGGC TGTCCTCCGC TTTGGCTTTC TACAGGAATT CTCCAAGGAG
421      GAGAGAGACC CTGTGAAAAC CCATGAGGGC TGGGGAGTGA TGCTGCCCTG TAACCCGCCT
481      GCCCATTACC CAGGTTTGTC CTACCGCTGG CTCCTCAACG AGTTCCCCAA CTTCATCCCA
541      ACGGATGGGC GACACTTTGT GTCCCAGACT ACAGGAAACC TGTACATCGC CCGGACCAAT
601      GCCTCAGACC TGGGCAACTA CTCTTGTTTG GCTACCAGCC ACATGGACTT TTCCACCAAG
661      AGTGTCTTCA GCAAATTTGC GCAGCTCAAC CTGGCTGCGG AAGATCCCCG ACTCTTCGCT
721      CCCAGTATCA AAGCTCGGTT CCCCCCGGAG ACCTACGCAC TAGTTGGGCA GCAAGTCACC
781      CTGGAGTGCT TTGCCTTTGG GAACCCGGTT CCCCGGATCA AGTGGCGCAA AGTGGATGGT
841      TCCTTGTCCC CTCAGTGGGC CACAGCTGAG CCCACCCTGC AGATCCCCAG CGTGAGCTTT
901      GAAGACGAGG GTACCTATGA ATGTGAGGCA GAGAACTCCA AGGGTCGTGA CACCGTCCAG
961      GGACGCATCA TCGTGCAAGC TCAGCCTGAG TGGCTAAAGG TGATCTCAGA CACAGAGGCC
1021     GACATTGGCT CCAACTTACG TTGGGGCTGT GCAGCAGCAG GCAAACCCCG GCCCATGGTG
1081     CGCTGGCTGA GAAACGGGGA ACCTCTGGCC TCCCAGAACC GGGTGGAGGT CTTGGCTGGG
1141     GACCTGCGAT TCTCTAAGCT GAGCCTGGAG GACTCTGGCA TGTACCAGTG TGTGGCTGAA
1201     AACAAGCATG GCACCATCTA TGCCAGTGCT GAGCTGGCTG TACAAGCTCT GGCCCCAGAC
1261     TTCAGGCAGA ACCCTGTGAG ACGGCTGATC CCTGCAGCTC GAGGCGGAGA GATCAGCATC
1321     CTGTGCCAGC CTCGCGCAGC CCCAAAAGCT ACAATACTTT GGAGCAAGGG TACTGAGATT
1381     TTGGGGAACA GTACCAGAGT GACTGTCACT TCCGATGGCA CCTTGATCAT CAGAAACATC
1441     AGCCGATCCG ATGAAGGCAA ATATACCTGC TTTGCTGAGA ACTTCATGGG CAAAGCCAAC
1501     AGTACCGGGA TCCTGTCCGT GCGCGATGCA ACCAAGATCA CCCTGGCTCC CTCCAGTGCT
1561     GACATCAACG TGGGTGACAA CCTGACCCTA CAATGTCATG CCTCGCACGA CCCCACTATG
1621     GACCTCACGT TCACCTGGAC CCTGGATGAT TTCCCTATTG ACTTTGATAA GCCTGGAGGT
1681     CACTACCGGA GAGCCAGTGC GAAGGAGACC ATTGGGGACC TGACTATCCT CAACGCCCAC
1741     GTACGCCATG GAGGGAAGTA CACATGCATG GCCCAGACTG TGGTAGATGG TACATCCAAG
1801     GAGGCCACAG TCCTGGTCCG AGGTCCCCCA GGTCCCCCAG GGGGTGTGGT GGTGAGAGAC
1861     ATCGGAGACA CCACCGTTCA GCTTAGCTGG AGTCGTGGCT TTGACAACCA CAGCCCCATT
1921     GCCAAGTACA CGCTGCAAGC TCGTACTCCA CCCTCGGGGA AATGGAAGCA GGTTCGGACC
1981     AATCCTGTGA ATATCGAGGG TAATGCCGAG ACTGCCCAGG TGCTGGGTCT CATGCCTTGG
2041     ATGGACTATG AGTTTCGGGT TTCAGCTAGC AACATCTTGG GCACTGGGGA GCCCAGCGGG
2101     CCCTCCAGCA AAATCCGCAC TAAGGAAGCA GTCCCCTCAG TGGCACCATC GGGACTCAGT
2161     GGAGGGGGAG GAGCCCCTGG AGAGCTCATC ATCAACTGGA CTCCCGTGTC ACGGGAGTAC
2221     CAGAACGGAG ACGGCTTCGG CTACCTGCTG TCCTTCCGCA GGCAAGGCAG CTCCAGCTGG
2281     CAGACTGCCC GGGTGCCTGG CGCTGATGCG CAGTACTTCG TCTACGGCAA TGACAGCATC
2341     CAGCCCTACA CACCCTTTGA GGTCAAGATC CGAAGCTACA ATCGCCGGGG GGATGGGCCC
2401     GAGAGCCTCA CTGCGTTAGT GTACTCAGCA GAGGAAGAGC CCAGGGTGGC CCCTGCCAAG
2461     GTCTGGGCCA AGGGGTCCTC ATCTTCAGAG ATGAACGTGA GCTGGGAGCC TGTGCTACAA
2521     GACATGAACG GCATTCTCCT GGGATATGAG ATTCGCTACT GGAAAGCCGG GGACAACGAA
2581     GCAGCCGCTG ACCGAGTGAG GACAGCAGGG CTAGACACCA GTGCCCGAGT CACTGGCCTG
2641     AACCCCAACA CCAAATACCA CGTAACTGTG AGGGCCTACA ACCGGGCCGG CACTGGACCC
2701     GCTAGCCCTT CAGCTGATGC CATGACCGTG AAGCCCCCGC CACGGAGACC TCCTGGCAAC
2761     ATCTCCTGGA CTTTCTCAAG CTCCAGTCTC AGCCTTAAGT GGGACCCTGT GGTTCCTCTC
2821     CGAAATGAAT CTACGGTCAC TGGCTACAAG ATGCTGTATC AGAATGATTT GCACCCAACT
2881     CCTACGCTCC ACCTCACCAG CAAGAACTGG ATAGAAATAC CAGTACCCGA AGACATTGGC
2941     CACGCTCTGG TACAGATTCG AACCACAGGG CCTGGAGGGG ATGGGATCCC CGCAGAAGTC
3001     CACATTGTGA GAAATGGAGG CACAAGCATG ATGGTGGAGA GCGCCGCCGC CCGCCCTGCC
3061     CATCCCGGAC CTGCGTTCTC CTGCATGGTG ATATTGATGC TCGCTGGCTA CCAGAAGCTC
3121     TGA

 

Quality Control:cDNA sequencing results is available upon request.