Rat CNTN1 Gene cDNA Clone / ORF Clone

CNTN1 cDNA Clone Datasheet

CNTN1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_057118.1
• cDNA Size: 3066
• Gene Synonym: F3; Cntn1
• Source: Rat

CNTN1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus contactin 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1584 C/G，2034 G/A，2457 C/A，2505 A/G and 2511 G/A not causing the amino acid variation.

CNTN1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CNTN1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CNTN1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAAACCC CATTGCTGGT CAGTCATCTC CTGCTAATCT CCTTAACATC CTGTCTAGGA
61       GAGTTTACAT GGCACAGAAG ATATGGTCAT GGAGTTTCTG AGGAGGATAA AGGATTTGGA
121      CCCATTTTTG AAGAGCAACC AATCAATACC ATTTACCCAG AAGAGTCACT GGAAGGCAAA
181      GTGTCGCTCA ACTGCCGGGC ACGGGCAAGC CCATTTCCGG TTTACAAGTG GAGAATGAAT
241      AACGGGGACG TTGACCTGAC GAACGATCGA TACAGCATGG TCGGAGGGAA CCTTGTTATC
301      AATAACCCTG ACAAACAGAA AGACGCAGGA ATATACTACT GCTTGGCGTC CAACAACTAC
361      GGGATGGTCA GAAGCACCGA GGCCACCCTG AGCTTTGGGT ATCTTGACCC CTTTCCTCCT
421      GAGGATCGTC CTGAAGTGAA GGTGAAGGAA GGGAAGGGCA TGGTGCTTCT CTGCGACCCT
481      CCCTACCACT TCCCAGACGA TCTTAGCTAC CGCTGGCTTC TGAATGAATT TCCCGTCTTT
541      ATCACAATGG ATAAGCGAAG ATTCGTGTCT CAGACCAACG GTAATCTCTA CATCGCGAAC
601      GTTGAGTCTT CTGATAGAGG CAACTATTCC TGCTTCGTGT CCAGCCCTTC TATTACAAAG
661      AGCGTGTTCA GCAAATTTAT ACCCCTCATT CCGATACCTG AGCGAACAAC GAAGCCATAT
721      CCTGCTGACA TTGTAGTGCA GTTCAAGGAC ATATACACCA TGATGGGCCA AAATGTGACC
781      TTAGAGTGCT TCGCTCTTGG AAACCCTGTT CCTGATATTC GGTGGCGGAA GGTGCTAGAA
841      CCCATGCCTA CCACAGCTGA GATCAGCACC TCAGGGGCTG TCCTCAAGAT CTTCAACATC
901      CAGCTAGAAG ATGAAGGGCT CTACGAGTGC GAGGCTGAGA ACATTAGGGG AAAGGACAAG
961      CACCAGGCAA GAATTTATGT TCAAGCATTT CCTGAATGGG TAGAGCATAT CAATGACACT
1021     GAAGTGGACA TAGGCAGTGA CCTCTACTGG CCTTGTGTGG CCACAGGGAA GCCCATCCCT
1081     ACCATCAGGT GGCTGAAAAA TGGGTACGCG TATCACAAAG GGGAACTGAG GCTGTATGAC
1141     GTGACTTTTG AAAACGCCGG GATGTACCAG TGCATAGCAG AAAACGCGTA TGGGACCATT
1201     TATGCAAATG CTGAACTCAA GATCCTGGCT TTAGCTCCAA CTTTTGAAAT GAATCCCATG
1261     AAGAAGAAGA TCCTGGCTGC GAAAGGAGGA AGGGTTATCA TTGAGTGCAA ACCCAAGGCT
1321     GCACCAAAAC CCAAGTTTTC ATGGAGCAAA GGGACAGAGT GGCTTGTCAA CAGCAGCAGA
1381     ATACTCATAT GGGAAGATGG TAGCTTGGAA ATCAATAATA TTACAAGAAA TGATGGAGGA
1441     ATCTATACAT GCTTTGCAGA AAACAACAGA GGGAAAGCTA ATAGCACCGG GACTCTGGTG
1501     ATCACAAATC CCACACGAAT CATTTTGGCC CCAATCAATG CTGATATCAC TGTTGGAGAA
1561     AACGCTACCA TGCAGTGTGC TGCGTCCTTT GATCCTTCCT TGGATCTCAC GTTCGTCTGG
1621     TCTTTCAATG GCTACGTGAT TGACTTTAAC AAGGAAATTA CAAATATTCA TTACCAGAGG
1681     AATTTTATGC TCGACGCCAA TGGGGAACTG CTCATCCGAA ACGCGCAGCT GAAACACGCA
1741     GGACGGTACA CGTGCACAGC GCAGACAATC GTGGACAATT CTTCAGCTTC AGCTGACCTT
1801     GTCGTACGAG GCCCCCCAGG CCCTCCAGGT GGCCTGAGAA TAGAAGACAT CAGAGCTACC
1861     TCTGTGGCAC TGACTTGGAG CCGTGGTTCA GACAATCACA GCCCTATCTC TAAATACACT
1921     ATCCAGACCA AGACGATTCT CTCAGATGAC TGGAAAGACG CAAAGACGGA TCCCCCAATC
1981     ATTGAAGGAA ACATGGAGTC AGCAAAAGCA GTCGATTTGA TCCCATGGAT GGAATACGAA
2041     TTTCGAGTGG TAGCAACCAA TACTTTGGGG ACTGGAGAGC CCAGCATACC CTCAAACAGG
2101     ATCAAAACCG ATGGCGCTGC ACCAAATGTG GCTCCCTCAG ACGTAGGTGG TGGGGGCGGA
2161     ACCAACAGAG AGCTGACAAT AACGTGGGCG CCTTTGTCCA GAGAATACCA TTATGGTAAC
2221     AATTTTGGTT ACATAGTGGC ATTCAAGCCA TTCGATGGAG AAGAGTGGAA GAAAGTGACC
2281     GTTACCAATC CGGATACTGG CCGCTACGTC CACAAGGACG AAACCATGAC CCCTTCCACC
2341     GCGTTTCAAG TCAAAGTGAA GGCATTCAAC AATAAAGGAG ACGGGCCCTA CAGCCTCATA
2401     GCTGTCATCA ACTCAGCACA AGACGCTCCC AGTGAAGCCC CCACAGAAGT AGGGGTAAAA
2461     GTGCTATCGT CCTCTGAGAT CTCTGTCCAT TGGAAGCACG TTCTGGAGAA AATTGTGGAA
2521     AGCTATCAAA TTCGGTACTG GGCCGGTCAC GACAAAGAAG CAGCGGCGCA CAGAGTTCAA
2581     GTCACCAGCC AGGAGTACTC AGCCAGGCTG GAGAACCTTC TGCCGGACAC TCAGTACTTC
2641     ATAGAGGTGG GAGCCTGTAA CAGCGCGGGG TGCGGACCTT CCAGCGACGT GATCGAGACC
2701     TTCACCAGAA AAGCCCCTCC CAGCCAACCA CCAAGGATCA TCAGTTCCGT GAGGTCTGGC
2761     TCCCGATACA TAATCACGTG GGATCATGTC GTGGCACTAT CCAATGAATC TACAGTGACA
2821     GGCTACAAGA TACTCTATAG ACCTGACGGC CAACATGACG GTAAGCTGTT CTCCACGCAC
2881     AAGCACTCCA TAGAAGTCCC CATCCCCAGA GACGGAGAGT ACGTAGTCGA AGTTCGAGCA
2941     CACAGTGATG GAGGAGATGG GGTAGTGTCC CAAGTCAAAA TTTCAGGTGT GTCCACGCTG
3001     TCATCGGGTC TCCTCAGCTT GCTCCTGCCC TCCCTTGGCT TTCTTGTCTT CTATTCGGAA
3061     TTCTGA

 

Quality Control:cDNA sequencing results is available upon request.