CNPY4 Protein (His Tag)

Canopy 4 homolog (zebrafish) Protein Datasheet

CNPY4 Protein Product Information

• Synonym: CNPY4
• Protein Construction: A DNA sequence encoding the human CNPY4 (Q8N129) (Met1-Leu248) was expressed with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

CNPY4 Protein QC Testing

• Purity: > 85% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gly 22
• Molecular Mass: The recombinant human CNPY4 consists of 238 amino acids and predicts a molecular mass of 27.4 KDa. It migrates as an approximately 32 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CNPY4 protein

CNPY4 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CNPY4 Protein Description

CNPY4 is a member of the canopy family. CNPY4 interacts with toll-like receptor 4 (TLR4) and plays a role in the regulation of the cell surface expression of TLR4. Toll-like receptors (TLRs) recognize microbial products and induce immune responses. As CNPY4, PRAT4B also regulates cell surface expression of TLR4. PRAT4B has a signal peptide followed by a mature peptide. It is associated with the hypoglycosylated, immature form of TLR4. Lipopolysaccharide is recognized by the receptor complex consisting of TLR4 and MD-2.

References

1. Stelzl U. et al., 2005, Cell. 122 (6): 957-68.
2. Hocking JC. et al., 2010, Mech Dev. 127 (1-2): 36-48.
3. Hart BE. et al., 2012, J Biol Chem. 287 (20): 16550-62.