|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of canopy 2 homolog (zebrafish) DNA.|
|UNQ1943/PRO4426, HP10390, MSAP, TMEM4, ZSIG9, CNPY2|
|Identical with the Gene Bank Ref. ID sequence.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human CNPY2 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG13771-G-F|
|Human CNPY2 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG13771-G-H|
|Human CNPY2 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG13771-G-M|
|Human CNPY2 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG13771-G-N|
|Human CNPY2 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG13771-G-Y|
|Product name||Product name|
CNPY2 is a novel MIR-interacting protein that enhances neurite outgrowth and increases myosin regulatory light chain. CNPY2 enhances migration of C6 glioma cells through phosphorylation of the myosin regulatory light chain. It is expressed in different tissues, including brain. Overexpression of CNPY2 enhanced the motility of glioma cells measured in matrigel invasion chambers and using a scratch assay. Downregulation of CNPY2 by RNA interference significantly decreased glioma cell migration and phosphorylation of MRLC. Inhibition of the corresponding MRLC kinase by ML-7 did not affect migration of CNPY2-overexpressing cells.