Mouse CNDP1 Protein (His Tag)

Carnosine dipeptidase 1 (metallopeptidase M20 family) Protein Datasheet

CNDP1 Protein Product Information

• Synonym: AI746433, Cn1
• Protein Construction: A DNA sequence encoding the mouse CNDP1 (Q8BUG2) (Met 1-Tyr 492) was expressed, with an N-terminal signal peptide and a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

CNDP1 Protein QC Testing

• Purity: > 93 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to cleave carnosine (ß-Ala-L-His)in a two step assay.
  The specific activity is > 250 pmoles/min/μg.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The secreted recombinant mouse CNDP1 comprises 503 amino acids and has a calculated molecular mass of 56.5 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 55 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CNDP1 protein

CNDP1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CNDP1 Protein Description

CNDP1, also known as glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1), is a member of the M20 metalloprotease family. This protein is a secreted homodimeric dipeptidase that specifically hydrolyzes L-carnosine, and is identified as human carnosinase expressed in the brain. The CN1 gene contains trinucleotide (CTG) repeat length polymorphism in the coding region, which has been demonstrated to be associated with susceptibility to developing diabetic nephropathy, for carnosine protects against the adverse effects of high glucose levels on renal cells. In addition, this enzyme is also suggested to be implicated in the actions of neuroprotection and neurotransmiting.

References

1. Teufel Mm. et al., 2004, J Biol Chem. 278: 6521-32.
2. Riedl E. et al., 2007, Diabetes. 56: 2410-3.
3. Sauerhofer S. et al., 2007, Diabetes. 56: 2425-32.