- EGFR Signaling Pathway
- TGF-beta Signaling
- Canonical Wnt Signaling
- non-Canonical Wnt Signaling
- Notch Signaling
- p53 Pathway
- NF-kB Pathway
- Cytokine Signaling
>Rabbit MAb Antibody
>Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Rabbit Mab
|Catalog||Size (Price)||Quantity||In Stock||Operation|
Human CMV Glycoprotein B / gB Antibody
|Order or Inquire for gB Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0.125 ng/lane in WB|
|Detection limit is 0.0049 ng/well in ELISA|
Human CMV Glycoprotein B / gB Antibody Product Information
|Antibody Type :||Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )|
Clone ID :
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.|
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human CMV gB ( Catalog#10202-VCCH1 ; AAA45920.1 ) extracellular domain ( Met 1- Lys 700 ) linked with the cytoplasmic domain (Arg 777- Val 907).
Human CMV Glycoprotein B / gB Antibody Usage Guide
Human CMV Glycoprotein B / gB
|Western blot :||This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CMV gB in WB. Using a DAB detection system, the detection limit for human CMV gB is approximately 0.125 ng/lane under non-reducing conditions and 100ng/lane under reducing conditions|
|Direct ELISA :||This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human CMV gB. The detection limit for gB is 0.0049 ng/well|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CMV Glycoprotein B / gB Antibody Related Products & Topics
|Source||Molecule||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|Cytomegalovirus (CMV)||Glycoprotein B (gB)||CMV gB Protein, Recombinant||10202-V08H1|
|Cytomegalovirus (CMV)||Glycoprotein B (gB)||CMV gB/Fc Protein, Recombinant||10202-V02H1|
|Cytomegalovirus (CMV)||Glycoprotein B (gB)||CMV gB ECD/Fc Protein, Recombinant||10202-V02H2|
|Cytomegalovirus (CMV)||Glycoprotein B (gB)||CMV gB Protein, Recombinant||10202-VCCH1|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|WB, ELISA||Mouse Monoclonal Antibody||10202-MM02|
|WB, ELISA||Rabbit Monoclonal Antibody||10202-R038|
|WB, ELISA||Rabbit Polyclonal Antibody (Antigen Affinity Purified)||10202-RP02|
Human CMV Glycoprotein B / gB Antibody Background
Human cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B is a 907-amino acid glycoprotein encoded by the ORF of UL55. It is the most abundant component of the envelope with at least two defined neutralizing epitopes, a target of neutralizing antibodies and an essential replication component. The synthesized gB precursor matures into a 130-160 kDa glycoprotein by acquiring N-linked glycosylation modifications, and is cleaved by the cellular protease furin into two components, a 93-116 kDa N-terminal fragment and a 55 kDa C-terminal fragment linked by a disulfide-bond, which is presented on the viral envelope as well as on the surface of virus-infected cells as a covalently associated homodimer. gB plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. It triggers penetration of cells and enhances the spread of infection in nonpolarized human fibroblasts. The interaction of gB with the non-heparin receptor CD209/DC-SIGN is the initiation of intracellular signaling and activation of the IFN-responsive pathway. In addition, gB is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.
- Kathleen A. et al., 1998, Journal of VirologY, Vol 72. No. 3: 1826-33.
- Sharof T, et al., 1999, Journal of virology, Vol. 73, No. 10: 8677-88.
- Pereira L, et al., 1984, Virology, 139(1):73-86.
- M. Reschke, et al., 1995, Journal of General Virology, 76: 113-22.
- Kathleen A. et al., 1999, Molecular and cellular biology, Vol. 19, No. 5: 3607-13.
- Robin L. et al., 1997, Journal of virology, Vol. 71, No. 12: 9803-07.