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Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Rabbit Mab

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gBAntibody Product Information
Antigen:Recombinant Human CMV gB / Glycoprotein B protein (Catalog#10202-VCCH1)
Clone ID:38
Ig Type:Rabbit IgG
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human CMV gB (Catalog#10202-VCCH1; AAA45920.1) extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907).
gBAntibody Usage Guide
Specificity:Human CMV Glycoprotein B / gB
Application:WB, ELISA

WB: 1-2 μg/mL

This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CMV gB in WB. Using a DAB detection system, the detection limit for Human CMV gB is approximately 0.125 ng/lane under non-reducing conditions and 100 ng/lane under reducing conditions.

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CMV gB. The detection limit for gB is 0.0049 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Background

Human cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 907-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.

References
  • Lopper M,et al. (2002). Disulfide bond configuration of human cytomegalovirus glycoprotein B. J Virol. 76(12): 6073-82.
  • Isaacson MK, et al. (2009) Human cytomegalovirus glycoprotein B is required for virus entry and cell-to-cell spread but not for virion attachment, assembly, or egress. J Virol. 83(8): 3891-903.
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