CMA1 / Chymase 1 Protein (His Tag)

Chymase 1, mast cell Protein Datasheet

CMA1 / chymase 1 Protein Product Information

• Synonym: CYH, MCT1, MGC119890, MGC119891, chymase 1
• Protein Construction: A DNA sequence encoding the human CMA1 (P23946) (Met 1- Asn 247) was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

CMA1 / chymase 1 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gly 20
• Molecular Mass: The secreted recombinant human CMA1 consists of 238 amino acids and predicts a molecular mass of 26.6 kDa. It migrates as an approximately 33KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH7.4, 10% gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CMA1 / chymase 1 protein

CMA1 / chymase 1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CMA1 / chymase 1 Protein Description

Chymase 1 (CMA1) is a chynotrypic serine proteinase belonging to the peptidase family S1. This proteinase is expressed in mast cells and thought to function in many fields, including the degradation of extracellular matrix, the generation of vasoactive peptides, the regulation of submucosal gland secretion, and the converting of angiotensinⅠto the vasoactive peptide angiotensinⅡ. In the normal state, chymase is stored in mast cells and has no enzymatic activity. Chymase is activated immediately upon its release into the extracellular matrix in vascular tissues after mast cells have been activated by stimuli such as vessel injury by grafting or a balloon catheter. In this case, chymase is responsible for converting angiotensinⅠto the angiotensinⅡ, and has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure.

References

1. Takai S. et al., 2003, Jpn J Pharmacol. 90 (3): 223-7.
2. Caughey GH. et al., 1993, Genomics. 15 (3): 614-20.
3. Urata H. et al., 1991, J Biol Chem. 266 (26): 17173-9.