CLM-9 / TREM4 / CD300LG Protein

CD300 molecule-like family member g Protein Datasheet

CLM-9 / TREM4 / CD300LG Protein Product Information

• Synonym: CD300LG, CLM9, TREM4
• Protein Construction: A DNA sequence encoding the human CD300LG (AAH25395.1)(Met1-Arg247) was expressed, fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Human
• Expression Host: Human Cells

CLM-9 / TREM4 / CD300LG Protein QC Testing

• Purity: (74 +24.5) % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 19
• Molecular Mass: The recombinant human CD300LG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 470 amino acids and has a predicted molecular mass of 51.7 kDa. The apparent molecular mass of the protein is approximately 70 and 34 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CLM-9 / TREM4 / CD300LG protein

CLM-9 / TREM4 / CD300LG Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CLM-9 / TREM4 / CD300LG Protein Description

CLM-9, also known as TREM4, is a member of the CD300 -like (CD300L) family. CLM-9 is widely expressed on hematopoietic cells. All CD300L proteins are type I cell surface glycoproteins that contain a single immunoglobulin (Ig) V-like domain.  It is a receptor which may mediate L-selectin-dependent lymphocyte rollings. CLM-9 binds SELL in a calcium dependent manner. CLM-9 also binds lymphocyte which suggests that it functions in lymphocyte adhesion. CLM-9 gene encodes the human and murine protein. The major CLM-9 transcript is expressed highly in human heart, skeletal muscle, and placenta. The mouse protein has been shown to be expressed in capillary endothelial cells. Human CLM-9 mediates the uptake of human IgA2 and mouse IgM.

References

1. Clark GJ. et al., 2009, Immunobiology. 214 (9-10): 730-6.
2. Engel K. et al., 2012, J Inherit Metab Dis. 35 (1): 133-40.
3. Lamesch P. et al., 2007, Genomics. 89 (3): 307-15.