Anti-CLIC4 Antibody

Cat: 12271-RP02
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Anti-CLIC4 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CLIC4 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human CLIC4
Immunogen
Recombinant Human CLIC4 protein (Catalog#12271-H07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human CLIC4 (rh CLIC4; Catalog#12271-H07E; Q9Y696-1; Ala 2-Lys 253). CLIC4 specific IgG was purified by Human CLIC4 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CLIC4 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CLIC4 Antibody (Rabbit Polyclonal antibody) Images
Human CLIC4 Immunoprecipitation(IP) 8131

CLIC4 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

2 µL anti-CLIC4 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CLIC4 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 29 kDa

Observed band size: 29 kDa

Human CLIC4 Western blot (WB) 13481

Anti-CLIC4 rabbit polyclonal antibody at 1:500 dilution

Lane A: Raji Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:29 kDa

Observed band size:29 kDa

(We are unsure as to the identity of these extra bands.)

Anti-CLIC4 Antibody Alternative Names
Anti-CLIC4L Antibody;Anti-H1 Antibody;Anti-huH1 Antibody;Anti-MTCLIC Antibody;Anti-p64H1 Antibody
CLIC4 Background Information

Chloride intracellular channel protein 4, also known as Intracellular chloride ion channel protein p64H1 and CLIC4, is a member of the chloride channel CLIC family. It contains one GST C-terminal domain. CLIC4 is a member of a family of intracellular chloride channels. It is regulated by p53, c-Myc, and tumor necrosis factor-alpha. CLIC4 is detected in epithelial cells from colon, esophagus and kidney (at protein level). CLIC4 has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. CLIC4 could promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Expression of CLIC4 is prominent in heart, kidney, placenta and skeletal muscle. Overexpression of CLIC4 in cancer cells inhibits tumor growth. Conversely, overexpression of CLIC4 in tumor stromal cells stimulates tumor growth. Thus, CLIC4 participates in normal and pathological processes and may serve as a useful target for therapies in disturbances of homeostasis and neoplastic transformation. Loss of CLIC4 in tumor cells and gain in tumor stroma is common to many human cancers and marks malignant progression. Up-regulation of CLIC4 in tumor stroma is coincident with myofibroblast conversion, generally a poor prognostic indicator. Reactivation and restoration of CLIC4 in tumor cells or the converse in tumor stromal cells could provide a novel approach to inhibit tumor growth.

Full Name
chloride intracellular channel 4
References
  • Ronnov-Jessen L., et al., 2002, Am. J. Pathol. 161:471-480.
  • Berryman M.A., et al., 2003, Cell Motil. Cytoskeleton 56:159-172.
  • Suh,K.S. et al., 2005, J Investig Dermatol Symp Proc. 10 (2):105-9.
  • Suh,K.S. et al., 2007, Mol Carcinog.46 (8):599-604.
  • Suh,K.S. et al., 2007, J Cell Sci  120 (Pt 15):2631-40.
  • Suh,K.S. et al., 2007, Clin Cancer Res  13 (1):121-31.
  • Product Description Host Clonality Application Catalog# (PDF)
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