CLIC4 Antibody (Antigen Affinity Purified)

CLIC4 Antibody ( Antigen Affinity Purified ) Datasheet

CLIC4 Antibody Product Information

• Immunogen: Recombinant Human CLIC4 protein (Catalog#12271-H07E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human CLIC4 (rh CLIC4; Catalog#12271-H07E; Q9Y696-1; Ala 2-Lys 253). CLIC4 specific IgG was purified by Human CLIC4 affinity chromatography.

CLIC4 Antibody Usage Guide

• Specificity: Human CLIC4
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CLIC4 in WB. Using a DAB detection system, the detection limit for Human CLIC4 is approximately 4 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CLIC4. The detection limit for Human CLIC4 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CLIC4 Antibody Background

Chloride intracellular channel protein 4, also known as Intracellular chloride ion channel protein p64H1 and CLIC4, is a member of the chloride channel CLIC family. It contains oneGST C-terminal domain. CLIC4 is a member of a family of intracellular chloride channels. It is regulated by p53, c-Myc, and tumor necrosis factor-alpha. CLIC4 is detected in epithelial cells from colon, esophagus and kidney (at protein level). CLIC4 has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. CLIC4 could promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Expression of CLIC4 is prominent in heart, kidney, placenta and skeletal muscle. Overexpression of CLIC4 in cancer cells inhibits tumor growth. Conversely, overexpression of CLIC4 in tumor stromal cells stimulates tumor growth. Thus, CLIC4 participates in normal and pathological processes and may serve as a useful target for therapies in disturbances of homeostasis and neoplastic transformation. Loss of CLIC4 in tumor cells and gain in tumor stroma is common to many human cancers and marks malignant progression. Up-regulation of CLIC4 in tumor stroma is coincident with myofibroblast conversion, generally a poor prognostic indicator. Reactivation and restoration of CLIC4 in tumor cells or the converse in tumor stromal cells could provide a novel approach to inhibit tumor growth.

References

1. Ronnov-Jessen L., et al., 2002, Am. J. Pathol. 161: 471-480.
2. Berryman M.A., et al., 2003, Cell Motil. Cytoskeleton 56: 159-172.
3. Suh,K.S. et al., 2005, J Investig Dermatol Symp Proc. 10 (2): 105-9.
4. Suh,K.S. et al.,2007, Mol Carcinog.46 (8): 599-604.
5. Suh,K.S. et al.,2007, J Cell Sci120 (Pt 15): 2631-40.
6. Suh,K.S. et al., 2007, Clin Cancer Res13 (1): 121-31.