Rat CLEC4F Gene cDNA Clone / ORF Clone

CLEC4F cDNA Clone Datasheet

CLEC4F cDNA Clone Product Information

• Gene Bank Ref.ID: NM_053753.1
• cDNA Size: 1653
• Gene Synonym: Kclr, Clecsf13, MGC112607, Clec4f
• Source: Rat

CLEC4F cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus C-type lectin domain family 4, member F DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1013 G/C and 1019 T/A resulting in the amino acid arg substitution by thr, leu substitution by gln.

CLEC4F cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CLEC4F cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CLEC4F cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGGAGG CGGAACTGAA CAGAGATGTG GCTAAATTCT GCACTGACAA CCAGTGTGTC
61       ATCCTACAGC CCCAAGGACT GGGGCCTAAG TCAGCTGCTC CCATGGCTCC CAGGACACTG
121      AGGCACGTCC AGGCCATTGT GGCACTAGTG GTGGTGACTG TGTTCTTCTC TCTCCTTGCT
181      CTTTTTGTGG TGGTTTTACA GCCTTGGAGA CAGAAGCAGA ATGAGGATCA TCCTGTCAAA
241      GCCGGGCTTC ATGGAGGCAA CTACTCTGGA TCTGACAATT GTTCCCAATT TGTCCGGAGG
301      GCAGAAATGC AAGAGGCTAT CCAGAGTCTC AGGGCCTCTG GGAATTCTAG CAGCTGTCAC
361      AAGGAGATAC AGACCCTGAA GTACCAGATG GACAATGTTA GTTCTCAGGT GCAGTTGCTT
421      GGGGGCCATC TGGAGGAAGC CAATGCTGAC ATCCAGCAGG CAAAGGATGT GCTGAAGGGC
481      ACTGGGGCCT TGGCCTCGGA GACCCAGGCA CTGAGAAGCT CCTTGGAGGT CGCCAGTGCG
541      GATATCCATA GTCTGAGAGG CGATTTGGAA AAGGCAAATG CCATGACTTC CCAGACCCAA
601      GGTCTCTTAA AGAGCAGCAC AGACAACACC AGTGCTGAGC TCCATGTGCT GGGCAGAGGC
661      CTGGAGGAAG CCCAGTCTGA GATCCAGGCT TTGAGGGGAA GTCTGCAGAG TTCAAATGAC
721      CTCGGCTCCC GGACTCAGAA CTTTCTCCAG CACAGTATGG ACAACATTAG TGCTGAAATC
781      CAGGCCATGA GAGATGGTAT GCAAAGAGCT GGCGAGGAGA TGACCTCATT AAAGAAAGAT
841      CTAGAAACAC TTACTGCTCA GATTCAAAAT GCAAATGGCC ACCTGGAGCA GACAGATACC
901      CAGATCCAGG GGCTAAAAGC TCAGTTGAAA AGCACCAGTT CCTTGAACTC CCAGATTGAG
961      GTGGTCAATG GCAAATTGAA AGACTCCAGC AGAGAGTTAC AGACACTGAG AACGGACCAG
1021     AGTGATGTCT CAGCTTTGAA GTCCAACGTC CAGATGTTAC AGAGCAATCT TCAGAAGGCC
1081     AAGGCAGAGG TACAGAGTTT GAAAACAGGT CTGGAGGCTA CCAAAACTCT CGCTGCCAAG
1141     ATTCAGGGAC AACAGAGTGA CCTGGAGGCC CTGCAGAAAG CTGTGGCTGC ACACACGCAG
1201     GGGCAGAAGA CTCAGAATCA GGTTCTCCAG CTGATCATGC AGGACTGGAA GTACTTCAAT
1261     GGAAAGTTTT ATTACTTTTC TCGTGACAAG AAGTCATGGC ATGAGGCCGA GAATTTCTGT
1321     GTGTCCCAGG GAGCACACCT GGCTTCGGTG ACCTCTCAGG AGGAGCAGGC ATTCCTGGTA
1381     CAGATCACAA ATGCTGTGGA CCATTGGATT GGGCTCACTG ATCAGGGCAC AGAGGGCAAC
1441     TGGCGCTGGG TGGATGGGAC ACCATTCGAT TATGTCCAGA GCAGAAGGTT TTGGAGGAAA
1501     GGCCAGCCTG ACAACTGGAG ACACGGAAAC GGAGAGCGTG AAGACTGTGT TCATCTGCAG
1561     CGGATGTGGA ACGACATGGC CTGTGGGACC GCCTACAACT GGGTGTGCAA GAAGTCTACA
1621     GATTGGTCTG TGGCCAGAAC GGACCAGAGC TGA

 

Quality Control:cDNA sequencing results is available upon request.