Human CLEC4E Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12227-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CLEC4E cDNA Clone Datasheet

CLEC4E cDNA Clone Product Information

Gene Bank Ref.ID:	BC000715
cDNA Size:	660
Gene Synonym:	MINCLE; CLECSF9;CLEC4E
Source:	Human

CLEC4E cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens C-type lectin domain family 4, member E DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CLEC4E cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CLEC4E cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CLEC4E cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAATTCAT CTAAATCATC TGAAACACAA TGCACAGAGA GAGGATGCTT CTCTTCCCAA
61       ATGTTCTTAT GGACTGTTGC TGGGATCCCC ATCCTATTTC TCAGTGCCTG TTTCATCACC
121      AGATGTGTTG TGACATTTCG CATCTTTCAA ACCTGTGATG AGAAAAAGTT TCAGCTACCT
181      GAGAATTTCA CAGAGCTCTC CTGCTACAAT TATGGATCAG GTTCAGTCAA GAATTGTTGT
241      CCATTGAACT GGGAATATTT TCAATCCAGC TGCTACTTCT TTTCTACTGA CACCATTTCC
301      TGGGCGTTAA GTTTAAAGAA CTGCTCAGCC ATGGGGGCTC ACCTGGTGGT TATCAACTCA
361      CAGGAGGAGC AGGAATTCCT TTCCTACAAG AAACCTAAAA TGAGAGAGTT TTTTATTGGA
421      CTGTCAGACC AGGTTGTCGA GGGTCAGTGG CAATGGGTGG ACGGCACACC TTTGACAAAG
481      TCTCTGAGCT TCTGGGATGT AGGGGAGCCC AACAACATAG CTACCCTGGA GGACTGTGCC
541      ACCATGAGAG ACTCTTCAAA CCCAAGGCAA AATTGGAATG ATGTAACCTG TTTCCTCAAT
601      TATTTTCGGA TTTGTGAAAT GGTAGGAATA AATCCTTTGA ACAAAGGAAA ATCTCTTTAA

Quality Control:cDNA sequencing results is available upon request.