Rat CLEC4D / CLECSF8 Protein (His Tag)

C-type lectin domain family 4, member Protein Datasheet

CLEC4D / CLECSF8 Protein Product Information

• Synonym: CLEC4D, Clecsf8, Mcl
• Protein Construction: A DNA sequence encoding the rat CLEC4D (Q69FH1) (Trp48-Lys218) was expressed, fused with a polyhistidine tag at the N-terminus.
• Source: Rat
• Expression Host: Human Cells

CLEC4D / CLECSF8 Protein QC Testing

• Purity: >88 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant rat CLEC4D is a disulfide-linked homodimer. The reduced monomer comprises 191 amino acids and has a predicted molecular mass of 22.3 kDa. The apparent molecular mass of the protein is approximately 29 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CLEC4D / CLECSF8 protein

CLEC4D / CLECSF8 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CLEC4D / CLECSF8 Protein Description

C-type lectin domain family 4 member D, also known as C-type lectin superfamily member 8, C-type lectin-like receptor 6, CLEC4D and CLECSF8, is a single-pass type I I membrane protein which contains one C-type lectin domain. CLEC4D is expressed weakly in peripheral blood leukocytes, bone marrow and spleen. Expression of CLEC4D is confined mostly in monocytes and macrophage and seems to be up-regulated by IL-6, IL-10, TNF-alpha and IFN-gamma. CLEC4D contains one C-type lectin domain. A C-type lectin (CLEC) is a type of carbohydrate-binding protein domain known as a lectin. The C-type designation is from their requirement for calcium for binding. Proteins that contain C-type lectin domains have a diverse range of functions including cell-cell adhesion, immune response to pathogens and apoptosis. CLEC4D functions as an endocytic receptor. It may be involved in antigen uptake at the site of infection, either for clearance of the antigen, or for processing and further presentation to T cells.

References

1. Drickamer K. et al., 1999, Curr Opin Struct Biol. 9 (5): 585-90.
2. Walker JR. et al., 2004, Biochemistry. 43 (13): 3783-92.
3. Zelensky AN. et al., 2005, FEBS J. 272 (24): 6179–217.
4. Cambi A. et al., 2009, Curr Biol. 19 (9): R375-8.