CLEC4D Antibody

CLEC4D Antibody Datasheet

CLEC4D Antibody Product Information

• Immunogen: Recombinant Human CLEC4D Protein (Catalog #11485-H07H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 001
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human CLEC4D (rh CLEC4D; Catalog #11485-H07H; Gly 52 - Asn 215; NP_525126.2).

CLEC4D Antibody Usage Guide

• Specificity: Human CLEC4D
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CLEC4D in WB. Using a DAB detection system, the detection limit for Human CLEC4D is approximately 1 ng/lane under non-reducing conditions and 0.25 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CLEC4D. The detection limit for Human CLEC4D is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CLEC4D Antibody Background

C-type lectin domain family 4 member D, also known as C-type lectin superfamily member 8, C-type lectin-like receptor 6, CLEC4D and CLECSF8, is a single-pass type I I membrane protein which contains one C-type lectin domain. CLEC4D is expressed weakly in peripheral blood leukocytes, bone marrow and spleen. Expression of CLEC4D is confined mostly in monocytes and macrophage and seems to be up-regulated by IL-6, IL-10, TNF-alpha and IFN-gamma. CLEC4D contains one C-type lectin domain. A C-type lectin (CLEC) is a type of carbohydrate-binding protein domain known as a lectin. The C-type designation is from their requirement for calcium for binding. Proteins that contain C-type lectin domains have a diverse range of functions including cell-cell adhesion, immune response to pathogens and apoptosis. CLEC4D functions as an endocytic receptor. It may be involved in antigen uptake at the site of infection, either for clearance of the antigen, or for processing and further presentation to T cells.

References

1. Drickamer K, et al., 1999, Curr. Opin. Struct. Biol. 9 (5): 585-90.
2. Walker JR, et al., 2004, Biochemistry 43 (13): 3783-92.
3. Zelensky AN, et al., 2005, FEBS J. 272 (24): 6179–217.
4. Cambi A, et al., 2009, Curr. Biol. 19 (9): R375-8.