Rat CLEC14A Gene cDNA Clone / ORF Clone

CLEC14A cDNA Clone Datasheet

CLEC14A cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001014077.1
• cDNA Size: 1407
• Gene Synonym: RGD1306232, Clec14a
• Source: Rat

CLEC14A cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus C-type lectin domain family 14, member A DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 998 A/G resulting in the amino acid gln substitution by arg.

CLEC14A cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CLEC14A cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CLEC14A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGCCGG CTCTTGCCCT GTGCCTCCTC TGTCCTGCGT TCTGGCCCCG GCCAGGGAAT
61       GGCGAGCACC CCACGGCCGA TCGCGCAGTT TGTTCGGTCC CGGGGGCTTG CTACAGCCTG
121      CACCACGCAG CCATGAAGAG GAGGGCGGCG GAGGAGGCCT GTAGCTTGCG CGGCGGGACT
181      CTCAGCACCG TGCAGTCCGG CTCTGAGTTG CAAGCTGTGC TCAAGCTCTT TCGTGCAGGT
241      CCCGGGCCTG GTGGAGGCTC CAAAGACCTA ATGTTCTGGG TGGCTCTGGA GCGTGACCGT
301      TCACAGTGCA CCCAGGAGAG AGAGCCTTTG AGGGGTTTCT CCTGGTTGTA CACGGACTCA
361      GAAGACTCGG AGAACAGAAC ACTACCTTGG GTGGAAGAGC CACAACTTTC TTGCACTGTA
421      CGAAAGTGCG CTGTGCTCCA GGCCACCGGG GGAGTGGAGC CTGCTGGCTG GAAGGAGATG
481      CGCTGTCAAC AGCGCGCCGA TGGCTACCTG TGCAAGTACC AGTTTGAGGC TCTGTGCCCT
541      GCGCCTCGCC CGGGAGCCGC CTCTAACTTA AGTTTCCAAG CTCCCTTCCG GCTGAGCAGT
601      TCTGCCCTGG ACTTCAGCCC TCCTGGGACC GAGGTGAGTG CGATGTGTCC CAGGGACTTC
661      TCAGTCTCCT CCATCTGCGT CCAGGAAGAG ACAGGCGCAC GCTGGGATGG GCTTTTCCCT
721      GGGAGCTTGC TCTGCCCCTG TTCTGGGAGG TACCTCCTTG CTGGCAAGTG CGTGGAGCTC
781      GCTGACTGCC TAGACTACTT GGGAAACTTC GCCTGCGAAT GTGCTGTGGG TTTTGAGCTA
841      GGGAAGGACA AACGCTCTTG TGAGACCAAA GCGGAAGGCC AACTAACCCT CGAGGGGACC
901      AAGTTGCCCA CTAGAAATGT ATCAGCCACT CCAGCCAGTC CTGTGACAAA CAAATCGTGG
961      CCAGGTCAGG TCTATGACAA GCCAGGAGAG ATGCCCCGGG TCACTGGACA AGACCGTATT
1021     GCGACATCTG TTCCTGAGAT TCTTCAGTGG GGAACACAGA GTACTTTACC TACTGTTCAA
1081     ACGTCCCCAC AAACCAAGCC AAAAGTCACC ATCACACCAT CAGGAAGTAT GATGCCCACA
1141     TTGAACTTCG CATCTTCCCC TCCTGTTTCT CTGACTTTCG ACTCCTCCTC CACGGTGGTC
1201     TTCGTACTTG TGAGCATAGC AGTAATAGTT TTGGTACTCT TGACCATTAC AGTGCTGGGA
1261     CTTTTCAAAC TCTGCTTCCA CAAAAGCCCT TCCTCCAGGA CAAGGAAGGG AGCTTTGGAC
1321     TCACAGGGTG TGGAATGTGA TGCAGAGGCC ACCTCTCTGC GGCACAATTC TGCACAGTGC
1381     ACGGACACTG GAGGGAAGGG TGCCTGA

 

Quality Control:cDNA sequencing results is available upon request.