Rat CLEC10A Gene cDNA Clone / ORF Clone

CLEC10A cDNA Clone Datasheet

CLEC10A cDNA Clone Product Information

• Gene Bank Ref.ID: NM_022393.2
• cDNA Size: 921
• Gene Synonym: Mgl, Mgl1, Mgll, Clec10a
• Source: Rat

CLEC10A cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus C-type lectin domain family 10, member A DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 635 C/T, 805 G/A resulting in the amino acid ala substitution by val, gly substitution by arg and 294 G/A，920 A/G，921 G/A not causing the amino acid variation.

CLEC10A cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CLEC10A cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CLEC10A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACAATGG CATATGAAAA CTTCCAGAAC TTGGGGAGCG AAGAGAAAAA CCAAGAGGCT
61       GGTAAAGCTC CTCCCCAGTC CTTCCTGTGC AATATCCTCT CTTGGACCCA CCTCCTCCTG
121      TTCTCCCTGG GCCTTAGCCT CCTGCTGCTG GTGGTTATCT CCGTGATTGG ATCCCAAAAT
181      TCCCAGTTAA GGAGGGACCT AGAAACCCTG AGAACCACTT TAGACAACAC CACCTCCAAC
241      ACCAAGGCTG AACTACAGGC CCTGGCCTCC AGGGGTGACA GCTTGCAAAC AGGAATCAAT
301      TCTCTGAAAG TGGAGGTGGA TGATCATGGG CAGGAACTGC AGGCAGGCCG AGGCTTGAGC
361      CAGAAGGTGG CTTCTCTGGA GAGCACAGTG GAGAAGAAGG AGCAGACTCT CAGAACAGAT
421      CTATCTGAAA TAACCGATCG TGTGCAACAG CTGGGGAAGG ACTTGAAGAC CCTGACATGT
481      CAGCTGGCCA GCCTCAAGAA CAACGGCTCA GCAGTGGCCT GCTGCCCCCT TCACTGGATG
541      GAGCATGAAG GCAGCTGCTA CTGGTTCTCT CAGTCTGGGA AGCCGTGGCC TGAAGCTGAC
601      AAGTACTGCC AGCTGGAGAA TTCTAACCTA GTGGTGGTCA ACTCCCTGGC GGAGCAGAAT
661      TTTCTACAGA CTCACATGGG CAGTGTGGTC ACTTGGATTG GCCTAACGGA CCAAAATGGG
721      CCCTGGCGAT GGGTGGACGG GACCGACTAT GAGAAAGGCT TTACGCACTG GGCCCCAAAG
781      CAGCCAGATA ACTGGTATGG ACACAGGCTG GGAGGAGGCG AGGACTGTGC ACACTTCACC
841      TCAGATGGTC GCTGGAATGA TGACGTTTGC CAGAGGCCCT ACCGCTGGGT CTGTGAGATG
901      AAGCTGGCCA AGGACAGCTG A

 

Quality Control:cDNA sequencing results is available upon request.