Human CLCF1 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10937-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_013246.2

cDNA Size:   678

Synonym:  CLC; NR6; BSF3; NNT1; CISS2

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens cardiotrophin-like cytokine factor 1 DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15–30 min .
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGACCTCC GAGCAGGGGA CTCGTGGGGG ATGTTAGCGT GCCTGTGCAC GGTGCTCTGG
61       CACCTCCCTG CAGTGCCAGC TCTCAATCGC ACAGGGGACC CAGGGCCTGG CCCCTCCATC
121      CAGAAAACCT ATGACCTCAC CCGCTACCTG GAGCACCAAC TCCGCAGCTT GGCTGGGACC
181      TATCTGAACT ACCTGGGCCC CCCTTTCAAC GAGCCAGACT TCAACCCTCC CCGCCTGGGG
241      GCAGAGACTC TGCCCAGGGC CACTGTTGAC TTGGAGGTGT GGCGAAGCCT CAATGACAAA
301      CTGCGGCTGA CCCAGAACTA CGAGGCCTAC AGCCACCTTC TGTGTTACTT GCGTGGCCTC
361      AACCGTCAGG CTGCCACTGC TGAGCTGCGC CGCAGCCTGG CCCACTTCTG CACCAGCCTC
421      CAGGGCCTGC TGGGCAGCAT TGCGGGCGTC ATGGCAGCTC TGGGCTACCC ACTGCCCCAG
481      CCGCTGCCTG GGACTGAACC CACTTGGACT CCTGGCCCTG CCCACAGTGA CTTCCTCCAG
541      AAGATGGACG ACTTCTGGCT GCTGAAGGAG CTGCAGACCT GGCTGTGGCG CTCGGCCAAG
601      GACTTCAACC GGCTCAAGAA GAAGATGCAG CCTCCAGCAG CTGCAGTCAC CCTGCACCTG
661      GGGGCTCATG GCTTCTGA

Quality Control: cDNA sequencing results is available upon request.

Cytokine