All CKM reagents are produced in house and quality controlled, including 4 CKM Antibody, 45 CKM Gene, 1 CKM Protein, 3 CKM qPCR. All CKM reagents are ready to use.
Recombinant CKM proteins are expressed by E. coli with fusion tags as N-His.
CKMantibodies are validated with different applications, which are ELISA, IHC-P.
CKMcDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each CKM of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
CKM, also known as CK-MM, is mainly expressed in skeletal muscle. As the primary CK isoenzyme, it also can be detected in heart muscle. CKM is a subunit of creatine kinase (CK). CK is an enzyme expressed by various tissues and cell types. It catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In the cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type).