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Human CIB2 ELISA Pair Set

  • Human CIB2 ELISA standard curve
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Human CIB2 Materials provided
Capture Ab:0.2 mg/mL of mouse anti-CIB2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 12596-MM09)
Detection Ab:0.5 mg/mL rabbit anti-CIB2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 12596-R001)
Standard:Each vial contains 14 ng of recombinant CIB2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.0 ng/mL is recommended.
Human CIB2 Sensitivity
The minimum detectable dose of Human CIB2 was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human CIB2 Principle of the product
The Human CIB2 ELISA Pair Set is for the quantitative determination of Human CIB2.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CIB2 coated on a 96-well plate. Standards and samples are added to the wells, and any CIB2 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-CIB2 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CIB2 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Human CIB2 Storage
Human CIB2 ELISA Pair Set Standard Curve
Human CIB2 References
  • Blazejczyk M, et al. (2006) Myristoylation and membranous localization of Calmyrin2, a new member of Neuronal Calcium-Sensor proteins. FENS Forum Abstracts. 3.
  • Hollenbach AD, et al. (2006) The EF-hand calcium-binding protein calmyrin inhibits the transcriptional and DNA-binding activity of Pax3. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1574(3): 321-8.
  • Blazejczyk M, et al. (2009) Biochemical characterization and expression analysis of a novel EF-hand Ca2+ binding protein calmyrin2 (Cib2) in brain indicates its function in NMDA receptor mediated Ca2+ signaling. Archives of Biochemistry and Biophysics. 487(1): 66-78.
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    Catalog: SEK12596-5
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