Human CHST12 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12308-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CHST12 cDNA Clone Datasheet

CHST12 cDNA Clone Product Information

Gene Bank Ref.ID:	BC002918
cDNA Size:	1245
Gene Synonym:	C4S-2; C4ST2; C4ST-2; CHST12
Source:	Human

CHST12 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens carbohydrate (chondroitin 4) sulfotransferase 12 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CHST12 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CHST12 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CHST12 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACCAAGG CCCGGCTGTT CCGGCTGTGG CTGGTGCTGG GGTCGGTGTT CATGATCCTG
61       CTGATCATCG TGTACTGGGA CAGCGCAGGC GCCGCGCACT TCTACTTGCA CACGTCCTTC
121      TCTAGGCCGC ACACGGGGCC GCCGCTGCCC ACGCCCGGGC CGGACAGGGA CAGGGAGCTC
181      ACGGCCGACT CCGATGTCGA CGAGTTTCTG GACAAGTTTC TCAGTGCTGG CGTGAAGCAG
241      AGCGACCTTC CCAGAAAGGA GACGGAGCAG CCGCCTGCGC CGGGGAGCAT GGAGGAGAGC
301      GTGAGAGGCT ACGACTGGTC CCCGCGCGAC GCCCGGCGCA GCCCAGACCA GGGCCGGCAG
361      CAGGCGGAGC GGAGGAGCGT GCTGCGGGGC TTCTGCGCCA ACTCCAGCCT GGCCTTCCCC
421      ACCAAGGAGC GCGCATTCGA CGACATCCCC AACTCGGAGC TGAGCCACCT GATCGTGGAC
481      GACCGGCACG GGGCCATCTA CTGCTACGTG CCCAAGGTGG CCTGCACCAA CTGGAAGCGC
541      GTGATGATCG TGCTGAGCGG AAGCCTGCTG CACCGCGGTG CGCCCTACCG CGACCCGCTG
601      CGCATCCCGC GCGAGCACGT GCACAACGCC AGCGCGCACC TGACCTTCAA CAAGTTCTGG
661      CGCCGCTACG GGAAGCTCTC CCGCCACCTC ATGAAGGTCA AGCTCAAGAA GTACACCAAG
721      TTCCTCTTCG TGCGCGACCC CTTCGTGCGC CTGATCTCCG CCTTCCGCAG CAAGTTCGAG
781      CTGGAGAACG AGGAGTTCTA CCGCAAGTTC GCCGTGCCCA TGCTGCGGCT GTACGCCAAC
841      CACACCAGCC TGCCCGCCTC GGCGCGCGAG GCCTTCCGCG CTGGCCTCAA GGTGTCCTTC
901      GCCAACTTCA TCCAGTACCT GCTGGACCCG CACACGGAGA AGCTGGCGCC CTTCAACGAG
961      CACTGGCGGC AGGTGTACCG CCTCTGCCAC CCGTGCCAGA TCGACTACGA CTTCGTGGGG
1021     AAGCTGGAGA CTCTGGACGA GGACGCCGCG CAGCTGCTGC AGCTACTCCA GGTGGACCGG
1081     CAGCTCCGCT TCCCCCCGAG CTACCGGAAC AGGACCGCCA GCAGCTGGGA GGAGGACTGG
1141     TTCGCCAAGA TCCCCCTGGC CTGGAGGCAG CAGCTGTATA AACTCTACGA GGCCGACTTT
1201     GTTCTCTTCG GCTACCCCAA GCCCGAAAAC CTCCTCCGAG ACTGA

Quality Control:cDNA sequencing results is available upon request.