Human CHST1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12306-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CHST1 cDNA Clone Datasheet

CHST1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC022567
cDNA Size:	1236
Gene Synonym:	C6ST; KSST; GST-1; KS6ST; KSGal6ST; CHST1
Source:	Human

CHST1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CHST1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CHST1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CHST1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAATGTT CCTGGAAGGC CGTCCTCCTC CTTGCCCTGG CCTCCATTGC CATCCAGTAC
61       ACGGCCATCC GCACCTTCAC CGCCAAGTCC TTTCACACCT GCCCCGGGCT GGCAGAGGCC
121      GGGCTGGCCG AGCGACTGTG CGAGGAGAGC CCCACCTTCG CCTACAACCT CTCCCGCAAG
181      ACCCACATCC TCATCCTGGC CACCACGCGC AGCGGCTCCT CCTTCGTGGG CCAGCTCTTC
241      AACCAGCACC TGGACGTCTT CTACCTGTTT GAGCCCCTCT ACCACGTCCA GAACACGCTC
301      ATCCCCCGCT TCACCCAGGG CAAGAGCCCG GCCGACCGGC GGGTCATGCT AGGCGCCAGC
361      CGCGACCTCC TGCGGAGCCT CTACGACTGC GACCTCTACT TCCTGGAGAA CTACATCAAG
421      CCGCCGCCGG TCAACCACAC CACCGACAGG ATCTTCCGCC GCGGGGCCAG CCGGGTCCTC
481      TGCTCCCGGC CTGTGTGCGA CCCTCCGGGG CCAGCCGACC TGGTCCTGGA GGAGGGGGAC
541      TGTGTGCGCA AGTGCGGGCT ACTCAACCTG ACCGTGGCGG CCGAGGCGTG CCGCGAGCGC
601      AGCCACGTGG CCATCAAGAC GGTGCGCGTG CCCGAGGTGA ACGACCTGCG CGCCCTGGTG
661      GAAGACCCGC GATTAAACCT CAAGGTCATC CAGCTGGTCC GAGACCCCCG CGGCATTCTG
721      GCTTCGCGCA GCGAGACCTT CCGCGACACG TACCGGCTCT GGCGGCTCTG GTACGGCACC
781      GGGAGGAAAC CCTACAACCT GGACGTGACG CAGCTGACCA CGGTGTGCGA GGACTTCTCC
841      AACTCCGTGT CCACCGGCCT CATGCGGCCC CCGTGGCTCA AGGGCAAGTA CATGTTGGTG
901      CGCTACGAGG ACCTGGCTCG GAACCCTATG AAGAAGACCG AGGAGATCTA CGGGTTCCTG
961      GGCATCCCGC TGGACAGCCA CGTGGCCCGC TGGATCCAGA ACAACACGCG GGGCGACCCC
1021     ACCCTGGGCA AGCACAAATA CGGCACCGTG CGAAACTCGG CGGCCACGGC CGAGAAGTGG
1081     CGCTTCCGCC TCTCCTACGA CATCGTGGCC TTTGCCCAGA ACGCCTGCCA GCAGGTGCTG
1141     GCCCAGCTGG GCTACAAGAT CGCCGCCTCG GAGGAGGAGC TGAAGAACCC CTCGGTCAGC
1201     CTGGTGGAGG AGCGGGACTT CCGCCCCTTC TCGTGA

Quality Control:cDNA sequencing results is available upon request.