Human CHRNA3 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12172-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CHRNA3 cDNA Clone Datasheet

CHRNA3 cDNA Clone Product Information

Gene Bank Ref.ID:	BC000513
cDNA Size:	1518
Gene Synonym:	LNCR2; PAOD2; NACHRA3;CHRNA3
Source:	Human

CHRNA3 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens cholinergic receptor, nicotinic, alpha 3 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CHRNA3 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CHRNA3 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CHRNA3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCTCTG GCCCGCTCTC GCTGCCCCTG GCGCTGTCGC CGCCGCGGCT GCTGCTGCTG
61       CTGCTGCTGT CTCTGCTGCC AGTGGCCAGG GCCTCAGAGG CTGAGCACCG TCTATTTGAG
121      CGGCTGTTTG AAGATTACAA TGAGATCATC CGGCCTGTAG CCAACGTGTC TGACCCAGTC
181      ATCATCCATT TCGAGGTGTC CATGTCTCAG CTGGTGAAGG TGGATGAAGT AAACCAGATC
241      ATGGAGACCA ACCTGTGGCT CAAGCAAATC TGGAATGACT ACAAGCTGAA GTGGAACCCC
301      TCTGACTATG GTGGGGCAGA GTTCATGCGT GTCCCTGCAC AGAAGATCTG GAAGCCAGAC
361      ATTGTGCTGT ATAACAATGC TGTTGGGGAT TTCCAGGTGG ACGACAAGAC CAAAGCCTTA
421      CTCAAGTACA CTGGGGAGGT GACTTGGATA CCTCCGGCCA TCTTTAAGAG CTCCTGTAAA
481      ATCGACGTGA CCTACTTCCC GTTTGATTAC CAAAACTGTA CCATGAAGTT CGGTTCCTGG
541      TCCTACGATA AGGCGAAAAT CGATCTGGTC CTGATCGGCT CTTCCATGAA CCTCAAGGAC
601      TATTGGGAGA GCGGCGAGTG GGCCATCATC AAAGCCCCAG GCTACAAACA CGACATCAAG
661      TACAACTGCT GCGAGGAGAT CTACCCCGAC ATCACATACT CGCTGTACAT CCGGCGCCTG
721      CCCTTGTTCT ACACCATCAA CCTCATCATC CCCTGCCTGC TCATCTCCTT CCTCACTGTG
781      CTCGTCTTCT ACCTGCCCTC CGACTGCGGT GAGAAGGTGA CCCTGTGCAT TTCTGTCCTC
841      CTCTCCCTGA CGGTGTTTCT CCTGGTGATC ACTGAGACCA TCCCTTCCAC CTCGCTGGTC
901      ATCCCCCTGA TTGGAGAGTA CCTCCTGTTC ACCATGATTT TTGTAACCTT GTCCATCGTC
961      ATCACCGTCT TCGTGCTCAA CGTGCACTAC AGAACCCCGA CGACACACAC AATGCCCTCA
1021     TGGGTGAAGA CTGTATTCTT GAACCTGCTC CCCAGGGTCA TGTTCATGAC CAGGCCAACA
1081     AGCAACGAGG GCAACGCTCA GAAGCCGAGG CCCCTCTACG GTGCCGAGCT CTCAAATCTG
1141     AATTGCTTCA GCCGCGCAGA GTCCAAAGGC TGCAAGGAGG GCTACCCCTG CCAGGACGGG
1201     ATGTGTGGTT ACTGCCACCA CCGCAGGATA AAAATCTCCA ATTTCAGTGC TAACCTCACG
1261     AGAAGCTCTA GTTCTGAATC TGTTGATGCT GTGCTGTCCC TCTCTGCTTT GTCACCAGAA
1321     ATCAAAGAAG CCATCCAAAG TGTCAAGTAT ATTGCTGAAA ATATGAAAGC ACAAAATGAA
1381     GCCAAAGAGA TTCAAGATGA TTGGAAGTAT GTTGCCATGG TGATTGATCG TATTTTTCTG
1441     TGGGTTTTCA CCCTGGTGTG CATTCTAGGG ACAGCAGGAT TGTTTCTGCA ACCCCTGATG
1501     GCCAGGGAAG ATGCATAA

Quality Control:cDNA sequencing results is available upon request.